RecombinantRabMAb

Recombinant Anti-Otx2 antibody [EPR3348] (ab92326)

Submit a review Q&A (4)References (1)
Western blot - Anti-Otx2 antibody [EPR3348] (ab92326)
  • Western blot - Anti-Otx2 antibody [EPR3348] (ab92326)
  • Anti-Otx2 antibody [EPR3348] (ab92326)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3348] to Otx2
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Otx2 antibody [EPR3348]
    See all Otx2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3348] to Otx2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Otx2 aa 250 to the C-terminus.

  • Positive control

    • Y79 cell lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.

    • Tissue specificity

      Expressed in brain.

    • Involvement in disease

      Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures.

    • Sequence similarities

      Belongs to the paired homeobox family. Bicoid subfamily.
      Contains 1 homeobox DNA-binding domain.

    • Developmental stage

      Embryo.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      • Alternative names

        • CPHD6 antibody
        • Homeobox protein OTX2 antibody
        • MCOPS 5 antibody
        • MCOPS5 antibody
        • MGC45000 antibody
        • Orthodenticle 2 antibody
        • Orthodenticle homeobox 2 antibody
        • Orthodenticle homolog 2 (Drosophila) antibody
        • Orthodenticle homolog 2 antibody
        • Orthodenticle2 antibody
        • Otx 2 antibody
        • otx2 antibody
        • OTX2_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Otx2 antibody [EPR3348] (ab92326)
        Western blot - Anti-Otx2 antibody [EPR3348] (ab92326)
        Anti-Otx2 antibody [EPR3348] (ab92326) at 1/1000 dilution (purified) + Y79 cell lysate at 10 µg

        Secondary
        HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

        Predicted band size: 32 kDa
        Observed band size: 32 kDa



        Blocking buffer: 5% NFDM/TBST
        Dilution buffer: 5% NFDM/TBST

      • Western blot - Anti-Otx2 antibody [EPR3348] (ab92326)
        Western blot - Anti-Otx2 antibody [EPR3348] (ab92326)
        Anti-Otx2 antibody [EPR3348] (ab92326) at 1/1000 dilution (unpurified) + Y79 cell lysate at 10 µg

        Secondary
        HRP labelled goat anti-rabbit IgG at 1/1000 dilution

        Predicted band size: 32 kDa

      • Anti-Otx2 antibody [EPR3348] (ab92326)
        Anti-Otx2 antibody [EPR3348] (ab92326)

      References (1)

      Publishing research using ab92326? Please let us know so that we can cite the reference in this datasheet.

      ab92326 has been referenced in 1 publication.

      • Bernard C  et al. A Mouse Model for Conditional Secretion of Specific Single-Chain Antibodies Provides Genetic Evidence for Regulation of Cortical Plasticity by a Non-cell Autonomous Homeoprotein Transcription Factor. PLoS Genet 12:e1006035 (2016). WB . PubMed: 27171438

      Customer reviews and Q&As

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      1-4 of 4 Abreviews or Q&A

      Question

      I have only been using ab21990 the order confirmation number is .   Please go ahead and send me ab76748 as a replacement. Thank you for sending time on resolving these issues.  

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number . To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

      Read More

      Question

      Here is the link to ab92515 ( https://www.abcam.com/Otx2-antibody-EPR3347-ab92515.html) The western has a single band close to the 25kD marker band.    While the ab21990 has two bands one just above 25kD and one at 37kD. Since 21990 detects both OTX1 (37kD) and OTX2 (32kD) I assume my lower band is OTX2. But if ab92515 detects both proteins then why don’t we see two bands?   Whereas ab76748 that you suggested previously doesn’t have any western images in the datasheet.   I am trying to estimate amounts of OTX2 only. And this is important hence all the questions.  

      Read More
      Answer

      Thank you for getting back to me with these questions.

      I have been under the assumption that you havwe been using ab21190 for your experiments, have you been using ab92515 as well?Based on sequence homology ab92515 will recognize both proteins. The absence of a second band in the image is unresolved but may be due to poor separation or extrememly low levels of Otx1 in the sample.

      Using ab21190 the lower band will be Otx2 which, in either isoform, has a lower molecular weight that Otx1. (http://www.uniprot.org/uniprot/P32243&http://www.uniprot.org/uniprot/P32242).

      Although ab76748 does not have any western blot images in the datasheet we have been assured that the immunogen used for this has no cross reactivity to Otx1.This product is guaranteed to work in western bloton ​human tissues and is covered by our Abpromise for a period of six months. Since we have been unable to resolve this problem, if you have purchased this product within the last 180 days, I would be happy to either send this replacement antibody or to process a refund. If you could please supply me with your Abcam order confirmation number for the purchase of ab21190 I can begin to process this replacement,

      Read More

      Question

      How about ab92515? Is there a reason you have not mention this Antibody – except that it is monoclonal and the others polyclonal? Does the immunogen cross react ? It has images that seem to indicate specificity to OTX2 only,  in a western.  

      Read More
      Answer

      Thank you for contacting us.

      The immunogen for ab92515 shares 100% identity with OTX1, so cross reactivity is predicted.


      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      Phoned because the target of ab21990 has changed and is now unsure about their data.

      Read More
      Answer

      Thank you for contacting us.

      I am sorry that you have found this product unsatisfactory following its recent target refinement. As we discussed on the phone the sequence for ab21990 is shared by both OTX1 and OTX2. It was because of this that we have changed the targets.

      We have other products which has been guaranteed to work in WB and ICH on human tissue. One such product is ab76748. According to information that I have recieved form the lab, this antibody is specific to Otx2 only with no cross reactivity with Otx1. The immunogenic peptide was selected from specific domains of Otx2.


      Another product I would like to mention is ab92326. The immunogen for this OTX2 antibody shares 81% identity with OTX1, so the antibody is not predicted to cross-react with OTX1. However, it is not guaranteed without testing

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Read More

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