Overview

  • Product name

    Anti-Ovalbumin antibody [1E7]
    See all Ovalbumin primary antibodies
  • Description

    Mouse monoclonal [1E7] to Ovalbumin
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Chicken
  • Immunogen

    Full length native protein (purified) corresponding to Chicken Ovalbumin. Full length native protein (purified), isolated from chicken egg white formaldehyde and lysine treated for 2 weeks at 35°C and then heat denatured by autoclaving for 1 h at 110°C.

  • Positive control

    • ELISA: recombinant protein

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Female CF1 x BALB/c mice were immunized i.p. with immunogen adsorbed onto Al(OH)3.
  • Clonality

    Monoclonal
  • Clone number

    1E7
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/8000. A strong reaction is seen in ELISA, with ovalbumin directly coated onto the microtiter well (1ug/ml) (See Koch et al reference).
WB 1/2000. Detects a band of approximately 45 kDa.

Target

  • Function

    Storage protein of egg white. Lack protease inhibitory activity.
  • Tissue specificity

    Major protein of egg white.
  • Sequence similarities

    Belongs to the serpin family. Ov-serpin subfamily.
  • Post-translational
    modifications

    The signal sequence is not cleaved. The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. The hydrophobic sequence which lies between residues 27 and 43 folds back on the preceding residues to form an amphipathic hairpin structure which is the signal element recognized by the membrane.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Allergen Gal d II antibody
    • Egg albumin antibody
    • OVAL_CHICK antibody
    • Ovalbumin antibody
    • Plakalbumin antibody
    • Serpin peptidase inhibitor antibody
    • SERPINB14 antibody
    see all

References

This product has been referenced in:

See all 3 Publications for this product

Customer reviews and Q&As

Answer



The monoclonal anti-ovalbumin ab17291 and ab17293 are issued from 2 different clones and the immunogen used to raise them is the full length native protein. Unfortunately the epitopes have not been mapped.

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