Overview

  • Product name
    Anti-Ovalbumin antibody [8C6]
    See all Ovalbumin primary antibodies
  • Description
    Mouse monoclonal [8C6] to Ovalbumin
  • Host species
    Mouse
  • Specificity
    Shows high affinity for Ovalbumin in its native form. It does not recognize denatured Ovalbumin.
  • Tested applications
    Suitable for: ELISAmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Chicken
  • Immunogen

    Purified chicken ovalbumin adsorbed onto aluminum hydroxide gel.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    8C6
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/6000.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Storage protein of egg white. Lack protease inhibitory activity.
    • Tissue specificity
      Major protein of egg white.
    • Sequence similarities
      Belongs to the serpin family. Ov-serpin subfamily.
    • Post-translational
      modifications
      The signal sequence is not cleaved. The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. The hydrophobic sequence which lies between residues 27 and 43 folds back on the preceding residues to form an amphipathic hairpin structure which is the signal element recognized by the membrane.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Allergen Gal d II antibody
      • Egg albumin antibody
      • OVAL_CHICK antibody
      • Ovalbumin antibody
      • Plakalbumin antibody
      • Serpin peptidase inhibitor antibody
      • SERPINB14 antibody
      see all

    References

    This product has been referenced in:
    • Richert LE  et al. A virus-like particle vaccine platform elicits heightened and hastened local lung mucosal antibody production after a single dose. Vaccine 30:3653-65 (2012). Read more (PubMed: 22465748) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Question
    Answer

    DISCOUNT CODE: ***

    Expiration date: August 17th, 2012

    I am very pleased to hear you would like to accept our offer and test ab17292 in Flow Cytometry. This code will give you: 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forFlow Cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Ab17291 and ab17293 are specific for denatured (heat, reduced or surface-bound) ovalbumin. Tested in inhibition ELISA, the two antibodies do not react with the exact same epitope, but are slightly overlapping. From the dilution guidelines, it is seen that ab17291 seems to be slightly stronger than the ab17293 in affinity. Ab17290 and ab17292 are specific for native ovalbumin. Tested in inhibition ELISA, the two antibodies do not react with the exact same epitope, but are slightly overlapping. From the dilution guidelines, it is seen that the ab17292 seems to be the antibody with the highest affinity. If you have any additional questions, please contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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