Product nameAnti-Ovalbumin antibody
See all Ovalbumin primary antibodies
DescriptionRabbit polyclonal to Ovalbumin
Tested applicationsSuitable for: IP, IHC-P, WB, ELISAmore details
Species reactivityReacts with: Chicken
Full length native protein (purified) corresponding to Chicken Ovalbumin aa 2-386. (Purified from Hen Egg White).
GSIGAASMEFCFDVFKELKVHHANENIFYCPIAIMSALAMVYLGAKDSTR TQINKVVRFDKLPGFGDSIEAQCGTSVNVHSSLRDILNQITKPNDVYSFS LASRLYAEERYPILPEYLQCVKELYRGGLEPINFQTAADQARELINSWVE SQTNGIIRNVLQPSSVDSQTAMVLVNAIVFKGLWEKAFKDEDTQAMPFRV TEQESKPVQMMYQIGLFRVASMASEKMKILELPFASGTMSMLVLLPDEVS GLEQLESIINFEKLTEWTSSNVMEERKIKVYLPRMKMEEKYNLTSVLMAM GITDVFSSSANLSGISSAESLKISQAVHAAHAEINEAGREVVGSAEAGVD AASVSEEFRADHPFLFCIKHIATNAVLFFGRCVSP
Database link: P01012
- Ovalbumin protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.27% Potassium phosphate, 0.88% Sodium chloride
Concentration information loading...
Purification notesab181688 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. ab181688 is sterile filtered.
Our Abpromise guarantee covers the use of ab181688 in the following tested applications.
|IHC-P||1/300 - 1/2000.|
|WB||1/500 - 1/2000. Predicted molecular weight: 43 kDa.|
|ELISA||1/5000 - 1/20000.|
FunctionStorage protein of egg white. Lack protease inhibitory activity.
Tissue specificityMajor protein of egg white.
Sequence similaritiesBelongs to the serpin family. Ov-serpin subfamily.
modificationsThe signal sequence is not cleaved. The functional signal for membrane translocation of ovalbumin becomes accessible when the nascent chain is 50 to 60 residues long. The hydrophobic sequence which lies between residues 27 and 43 folds back on the preceding residues to form an amphipathic hairpin structure which is the signal element recognized by the membrane.
- Information by UniProt
- Allergen Gal d II antibody
- Egg albumin antibody
- OVAL_CHICK antibody
Anti-Ovalbumin antibody (ab181688) at 1/1000 dilution (overnight at 4°C) + Reduced Ovalbumin at 0.005 µg
Peroxidase rabbit secondary antibody
30 min at RT at 1/40000 dilution
Predicted band size: 43 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
All lanes : Anti-Ovalbumin antibody (ab181688) at 1/5000 dilution
Lanes 1 & 3 : Ovalbumin protein at 1 µg
Lanes 2 & 4 : Ovalbumin protein at 0.25 µg
All lanes : Atto 425 conjugated goat anti rabbit secondary antibody at 1/10000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Lane 1 + 2 under reducing conditions; Lane 3 + 4 under non-reducing conditions.
ab181688 has been referenced in 3 publications.
- Sonzogni AS et al. Effect of Delivery Platforms Structure on the Epidermal Antigen Transport for Topical Vaccination. Biomacromolecules N/A:N/A (2018). PubMed: 30376297
- O'Dea S et al. Vector-free intracellular delivery by reversible permeabilization. PLoS One 12:e0174779 (2017). PubMed: 28358921
- Guest IC & Sell S Bronchial lesions of mouse model of asthma are preceded by immune complex vasculitis and induced bronchial associated lymphoid tissue (iBALT). Lab Invest 95:886-902 (2015). PubMed: 26006019