Overview

  • Product name

  • Description

    Rabbit polyclonal to OVGP1/OGP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Cow, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human OVGP1/OGP (N terminal).
    (Peptide available as ab188397)

  • Positive control

    • WB: 3T3 cell lysate IHC-P: Human fallopian tube
  • General notes

     This product was previously labelled as OVGP1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab118590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 79 kDa.
IHC-P Use a concentration of 5 µg/ml.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development.
  • Tissue specificity

    Oviduct.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 18 family.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle. Secretory granules.
  • Information by UniProt
  • Database links

  • Alternative names

    • CHIT5 antibody
    • EGP antibody
    • Estrogen dependent oviduct protein antibody
    • Estrogen-dependent oviduct protein antibody
    • MUC9 antibody
    • mucin 9 antibody
    • Mucin-9 antibody
    • OGP antibody
    • OVGP 9 antibody
    • Ovgp1 antibody
    • OVGP1_HUMAN antibody
    • Oviduct glycoprotein antibody
    • Oviduct-specific glycoprotein antibody
    • Oviductal glycoprotein antibody
    • Oviductin antibody
    see all

Images

  • ab118590 staining OVGP1/OGP in Cow oviduct tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 5% serum for 30 minutes at 21°C. Samples were incubated with primary antibody (1/400 in 1% BSA + PBST (PBS + 0.2% Tween)) for 1 hour at 21°C. A HRP-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.

    See Abreview

  • ab118590, at 5 µg/ml, staining OVGP1/OGP in formalin fixed, paraffin embedded Human fallopian tube by Immunohistochemistry.

References

This product has been referenced in:

  • Chen S  et al. Long-term culture of primary porcine oviduct epithelial cells: validation of a comprehensive in vitro model for reproductive science. Theriogenology 80:862-9 (2013). Read more (PubMed: 23973051) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (Bovine oviductal epithelial cell culture (BOEC))
Permeabilization
No
Specification
Bovine oviductal epithelial cell culture (BOEC)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Jan 16 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Sample
Cow Tissue sections (Oviduct)
Specification
Oviduct
Permeabilization
No
Fixative
Acetone

Abcam user community

Verified customer

Submitted Dec 17 2013

Answer

Thank you for confirming this information. I am pleased to help answer your questions:

1. How much volume is in the vial?
From the datasheet: We sell 50 ug per vial at 1 mg ml.
Can work out the volume using regular laboratory maths:

At 1mg/ml 1000 ul would contain 1000 ug of antibody

So for 50 ug of antibody:
1000 ug / 50 ug = 20
1000 ul / 20 = 50 ul of antibody solution

2. How many ml we can made from this vial
This depends on what dilution you use for your experiments.

The suggested starting concentration for the IHC with IHC-P human tissues is 5 ug/ml. This antibody is sold at a concentration of 1 mg/ml so a 1:200 dilution of the antibody stock solution would be the equivalent of 5 ug/ml. However, the dilution of antibody used can often require individual optimization, so please note that this is a guideline only.

3. Which is a suitable antigen retrieval method for this antibody?
The following method has been used in testing. However, again please be aware that this is a guidelineand may require some individual optimization.

Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C 20 minutes
Remove from heat and let stand at room temperature in buffer 20 minutes
Rinse in 1X TBS with Tween (TBST)1 minute @ RT

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact me.

Read More

Answer

Thank you for your inquiry.

I can confirm that one vial of contains 50ug with a concentration of 1mg/ml.

As a starting working solution for WB we recommend a concentration of 1ug/ml that is a 1:1000 dilution.

For IHC-P we recommend 5ug/ml as working solution, that is a 1:200 dilution.

These recommendations are not absolute and the scientist always has to optimize the dilutions. Especially for IHC we recommend to test two different dilutions first and then adjust according to the results.

Our extensive protocol page can be found when following this link:

https://www.abcam.com/index.html?pageconfig=popular_protocols

If your customer need a more specific advise, please feel free to contact me again with more information about the planned experiment.

I hope this information is helpful and wish your customer good luck with their research.

Read More

Answer

Thank you for your enquiry.

I can confirm that each vial of this antibody contains 50 ug of antibody. The concentration of the antibdoy is 1 mg/ml (1000ug/ml or 1ug/ul).

This is stated on the datasheet as 50 ug at 1 mg/ml

Therefore each vial contains: 20 ul of antibody

For IHC-P, we recommend a dilution of 5 ug/ml.
If the starting concentration of the antibody is 1000 ug/ml. then you can work out the dilution required using regular laboratory calculations:

1000 ug / 5 ug = 1:200 dilution

So, in 1 ml (1000 ul) of antibody solution for IHC-P, you will require 5 ul of antibody:
1000 ul / 200 = 5 ul

I can suggest that this is a recommended dilution only, and your individual experiment may require some individual optimization.

For antigen retreival, we would usually recommend to start with citrate buffer heat mediate antigen retreival. However, this may require some further optimization, and there are other antigen retreival methods available, such as high pH EDTA and enzymatic methods..

I hope this will be helpful to you. if you have any further questions, please do not hesitate to contact us.

Read More

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