Key features and details
- Sensitivity: 2.5 pg/ml
- Range: 2.05 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Sheep
Product nameOvine IL-12 p70 ELISA Kit
See all IL-12 p70 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, EDTA Plasma
Assay typeSandwich (quantitative)
Range2.05 pg/ml - 200 pg/ml
Sample specific recovery Sample type Average % Range Serum 72.35 68% - 79% Plasma 74.38 68% - 79% Cell culture media 94.78 80% - 114%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Sheep
Ovine IL-12 p70 ELISA Kit (ab273255) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Ovine IL-12 p70 in serum, plasma (Collect plasma using EDTA as an anticoagulant. Heparin and citrate are not recommended) and cell culture supernatants.
This assay employs an antibody specific for Ovine IL-12 p70 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-12 p70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Ovine IL-12 p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-12 p70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Ovine IL-12 p70 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Ovine IL-12 p70 Antibody 2 vials Ovine IL-12 p70 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
RelevanceIL-12 p70 is a disulfide bonded heterodimer of the IL-12 40 kDa and 35 kDa subunits.
- CLMF p35
Our Abpromise guarantee covers the use of ab273255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab273255 has not yet been referenced specifically in any publications.