Overview

  • Product name

    Anti-OVOL1 antibody [460C1a]
    See all OVOL1 primary antibodies
  • Description

    Mouse monoclonal [460C1a] to OVOL1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Dot blotmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment from the N-terminal of human OVOL1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    ab74520 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and then filtered through a 0.22µm membrane.
  • Clonality

    Monoclonal
  • Clone number

    460C1a
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 30 kDa.
Dot blot Use at an assay dependent dilution.

Target

  • Function

    Putative transcription factor. Involved in hair formation and spermatogenesis. May function in the differentiation and/or maintenance of the urogenital system.
  • Tissue specificity

    Expressed in fetal kidney, and also in adult pancreas and placenta. Not expressed in intestine, peripheral blood lymphocytes and ovary.
  • Sequence similarities

    Contains 4 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • hOvo1 antibody
    • Ovo homolog like 1 antibody
    • Ovo like 1(Drosophila) antibody
    • Ovo, Drosophila, homolog-like, 1 antibody
    • Ovol1 antibody
    • OVOL1_HUMAN antibody
    • Putative transcription factor Ovo like 1 antibody
    • Putative transcription factor Ovo-like 1 antibody
    see all

Images

  • Anti-OVOL1 antibody [460C1a] (ab74520) + Immunising recombinant peptide

    Predicted band size: 30 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?



    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

References

This product has been referenced in:

  • Tsuji G  et al. Aryl hydrocarbon receptor activation restores filaggrin expression via OVOL1 in atopic dermatitis. Cell Death Dis 8:e2931 (2017). Read more (PubMed: 28703805) »
See 1 Publication for this product

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