Key features and details
- Mouse monoclonal [460C1a] to OVOL1
- Suitable for: WB, Dot blot
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-OVOL1 antibody [460C1a]
See all OVOL1 primary antibodies
DescriptionMouse monoclonal [460C1a] to OVOL1
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment from the N-terminal of human OVOL1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab74520 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and then filtered through a 0.22µm membrane.
Our Abpromise guarantee covers the use of ab74520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 30 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionPutative transcription factor. Involved in hair formation and spermatogenesis. May function in the differentiation and/or maintenance of the urogenital system.
Tissue specificityExpressed in fetal kidney, and also in adult pancreas and placenta. Not expressed in intestine, peripheral blood lymphocytes and ovary.
Sequence similaritiesContains 4 C2H2-type zinc fingers.
- Information by UniProt
- hOvo1 antibody
- Ovo homolog like 1 antibody
- Ovo like 1(Drosophila) antibody
Anti-OVOL1 antibody [460C1a] (ab74520) + Immunising recombinant peptide
Predicted band size: 30 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
ab74520 has been referenced in 1 publication.
- Tsuji G et al. Aryl hydrocarbon receptor activation restores filaggrin expression via OVOL1 in atopic dermatitis. Cell Death Dis 8:e2931 (2017). PubMed: 28703805