Key features and details
- Rabbit polyclonal to OVOL1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OVOL1 antibody
See all OVOL1 primary antibodies
DescriptionRabbit polyclonal to OVOL1
Specificityab65023 detects endogenous levels of total OVOL1 protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide from N terminal of human OVOL1.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).|
FunctionPutative transcription factor. Involved in hair formation and spermatogenesis. May function in the differentiation and/or maintenance of the urogenital system.
Tissue specificityExpressed in fetal kidney, and also in adult pancreas and placenta. Not expressed in intestine, peripheral blood lymphocytes and ovary.
Sequence similaritiesContains 4 C2H2-type zinc fingers.
- Information by UniProt
- hOvo1 antibody
- Ovo homolog like 1 antibody
- Ovo like 1(Drosophila) antibody
All lanes : Anti-OVOL1 antibody (ab65023) at 1/500 dilution
Lane 1 : K562 cell extract at 30 µg
Lane 2 : A549 cell extract at 30 µg
Lane 3 : A549 cell extract with immunising peptide at 10 µg
Predicted band size: 30 kDa
Observed band size: 30 kDa
Additional bands at: 85 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
ab65023 has been referenced in 2 publications.
- Sajini AA et al. Loss of 5-methylcytosine alters the biogenesis of vault-derived small RNAs to coordinate epidermal differentiation. Nat Commun 10:2550 (2019). PubMed: 31186410
- Vetere A et al. OVO homologue-like 1 (Ovol1) transcription factor: a novel target of neurogenin-3 in rodent pancreas. Diabetologia 53:115-22 (2010). WB ; Rat . PubMed: 19882138