Overview

  • Product name

    Anti-OX40L/TNFSF4 antibody [EP1168Y]
    See all OX40L/TNFSF4 primary antibodies
  • Description

    Rabbit monoclonal [EP1168Y] to OX40L/TNFSF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD252/OX40L (C terminal). The exact sequence is proprietary.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab76130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
    • Involvement in disease

      Genetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance.
    • Sequence similarities

      Belongs to the tumor necrosis factor family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CD 134L antibody
      • CD 252 antibody
      • CD134 ligand antibody
      • CD134L antibody
      • CD252 antibody
      • CD252 antigen antibody
      • glycoprotein 34 kd antibody
      • Glycoprotein Gp34 antibody
      • GP34 antibody
      • OX-40L antibody
      • OX40 antigen ligand antibody
      • OX40 ligand antibody
      • OX40L antibody
      • TAX transcriptionally-activated glycoprotein 1 antibody
      • TNFL4_HUMAN antibody
      • Tnfsf4 antibody
      • Tumor necrosis factor (ligand) superfamily member 4 antibody
      • Tumor necrosis factor ligand superfamily member 4 antibody
      • TXGP1 antibody
      see all

    Images

    • All lanes : Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130) at 1/1000 dilution

      Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
      Lane 2 : A-172 (human brain glioblastoma) whole cell lysate
      Lane 3 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 30 kDa
      Observed band size: 30 kDa


      Exposure time: 6 seconds


      OX40L/TNFSF4 is a glycoprotein. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8642328)

       

      Negative control: Raji (PMID: 8642328)

    • All lanes : Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130) at 1/50000 dilution

      Lane 1 : recombinant CD252 / OX40L at 0.01 µg
      Lane 2 : recombinant CD252 / OX40L at 0.005 µg

      Secondary
      All lanes : goat anti-rabbit-HRP at 1/1000 dilution

      Predicted band size: 30 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?



      The recombinant protein was expressed as a fusion protein.

    References

    This product has been referenced in:

    • Wang X  et al. Effect of cyclosporin A intervention on the immunological mechanisms of coronary heart disease and restenosis. Exp Ther Med 12:3242-3248 (2016). IHC-P ; Rabbit . Read more (PubMed: 27882144) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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