Product nameAnti-OX40L/TNFSF4 antibody [EP1168Y]
See all OX40L/TNFSF4 primary antibodies
DescriptionRabbit monoclonal [EP1168Y] to OX40L/TNFSF4
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human CD252/OX40L (C terminal). The exact sequence is proprietary.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 30 kDa).|
FunctionCytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production.
Involvement in diseaseGenetic variations in TNFSF4 influence susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=The upstream region of TNFSF4 contains a single risk haplotype for SLE, which is correlated with increased expression of both cell-surface TNFSF4 and TNFSF4 transcripts. Increased levels of TNFSF4 are thought to augment T cell-APC interaction and the functional consequences of T cell activation, thereby destabilizing peripheral tolerance.
Sequence similaritiesBelongs to the tumor necrosis factor family.
- Information by UniProt
- CD 134L antibody
- CD 252 antibody
- CD134 ligand antibody
All lanes : Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : A-172 (human brain glioblastoma) whole cell lysate
Lane 3 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
Exposure time: 6 seconds
OX40L/TNFSF4 is a glycoprotein. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 8642328)
Negative control: Raji (PMID: 8642328)
All lanes : Anti-OX40L/TNFSF4 antibody [EP1168Y] (ab76130) at 1/50000 dilution
Lane 1 : recombinant CD252 / OX40L at 0.01 µg
Lane 2 : recombinant CD252 / OX40L at 0.005 µg
All lanes : goat anti-rabbit-HRP at 1/1000 dilution
Predicted band size: 30 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
The recombinant protein was expressed as a fusion protein.