Overview

  • Product name

    Oxalate Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Urine, Serum, Plasma, Tissue
  • Assay type

    Quantitative
  • Sensitivity

    < 20 µM
  • Assay time

    2h 20m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Oxalate Assay kit (Colorimetric) (ab196990) is an easy-to-use, sensitive and high throughput adaptable kit. In this assay, Oxalate reacts with oxalate converter and oxalate enzyme mix to form an intermediate that reacts in turn with a highly specific probe to generate color that can be detected at OD=450 nm.


    This product can detect Oxalate levels lower than 20 μM.


    Oxalate assay protocol summary:
    - add samples and standards to wells
    - add oxalate convertor and incubate for 1 hr
    - add reaction mix and incubate for 1 hr
    - analyze with microplate reader

  • Notes

    Oxalate (C2O42-), in the form of Oxalic acid is present in many foods and beverages (e.g. spinach, tea etc.). It accumulates in many plant tissues and play role in regulating pH, osmosis and calcium storage. In animals, oxalate is either absorbed from dietary intake or produced from glycolate metabolism in liver. Under normal conditions, the daily oxalate load can be excreted by kidney. However, hereditary defects can cause an increased level of oxalate, which leads to hyperoxaluria and results in the formation of kidney stones. Therefore, measurement of oxalate level is useful for the prevention, diagnosis and monitoring of kidney stones.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Oxalate Assay Buffer 1 x 25ml
    Oxalate Converter 1 x 0.2ml
    Oxalate Development Buffer 1 x 15ml
    Oxalate Enzyme Mix 1 vial
    Oxalate Probe 1 vial
    Oxalate Standard 1 vial

Images

  • 8 weeks old mice were placed for 10 weeks on special egg-white based diets: HPC diet (High phosphate and calcium diet; 20% lactate, 2% calcium, 1.25% phosphate); HP diet (High phosphate diet; 0.6% calcium, 1.20% phosphate); CO diet (Control diet; 0.6% calcium, 0.3% phosphate); WT: wild type; Npt2a: Npt2a-/- mice. Oxylate was measured using 196990
  • Oxalate concentration in normal human urine (3 donors, 10 μL sample), normal human serum (10 μL), Kiwi lysate (10 μL) and Spinach lysate (10 μL).

  • Oxalate Standard Curve.

Protocols

References

This product has been referenced in:

  • Zhu J  et al. Inhibiting inflammation and modulating oxidative stress in oxalate-induced nephrolithiasis with the Nrf2 activator dimethyl fumarate. Free Radic Biol Med 134:9-22 (2018). Read more (PubMed: 30599261) »
  • Li Y  et al. Response of Npt2a knockout mice to dietary calcium and phosphorus. PLoS One 12:e0176232 (2017). Mouse . Read more (PubMed: 28448530) »
See all 2 Publications for this product

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