Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 20 min
- Sample type: Plasma, Serum, Tissue, Urine
- Sensitivity: 20 µM
Product nameOxalate Assay Kit (Colorimetric)
Sample typeUrine, Serum, Plasma, Tissue
Sensitivity< 20 µM
Assay time2h 20m
Species reactivityReacts with: Mammals, Other species
Oxalate Assay kit (Colorimetric) (ab196990) is an easy-to-use, sensitive and high throughput adaptable kit. In this assay, Oxalate reacts with oxalate converter and oxalate enzyme mix to form an intermediate that reacts in turn with a highly specific probe to generate color that can be detected at OD=450 nm.
This product can detect Oxalate levels lower than 20 μM.
Oxalate assay protocol summary:
- add samples and standards to wells
- add oxalate convertor and incubate for 1 hr
- add reaction mix and incubate for 1 hr
- analyze with microplate reader
Oxalate (C2O42-), in the form of Oxalic acid is present in many foods and beverages (e.g. spinach, tea etc.). It accumulates in many plant tissues and play role in regulating pH, osmosis and calcium storage. In animals, oxalate is either absorbed from dietary intake or produced from glycolate metabolism in liver. Under normal conditions, the daily oxalate load can be excreted by kidney. However, hereditary defects can cause an increased level of oxalate, which leads to hyperoxaluria and results in the formation of kidney stones. Therefore, measurement of oxalate level is useful for the prevention, diagnosis and monitoring of kidney stones.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Oxalate Assay Buffer 1 x 25ml Oxalate Converter 1 x 0.2ml Oxalate Development Buffer 1 x 15ml Oxalate Enzyme Mix 1 vial Oxalate Probe 1 vial Oxalate Standard 1 vial
8 weeks old mice were placed for 10 weeks on special egg-white based diets: HPC diet (High phosphate and calcium diet; 20% lactate, 2% calcium, 1.25% phosphate); HP diet (High phosphate diet; 0.6% calcium, 1.20% phosphate); CO diet (Control diet; 0.6% calcium, 0.3% phosphate); WT: wild type; Npt2a: Npt2a-/- mice. Oxylate was measured using 196990
Oxalate concentration in normal human urine (3 donors, 10 μL sample), normal human serum (10 μL), Kiwi lysate (10 μL) and Spinach lysate (10 μL).
Oxalate Standard Curve.
ab196990 has been referenced in 4 publications.
- Wu Y et al. Resveratrol Attenuates Oxalate-Induced Renal Oxidative Injury and Calcium Oxalate Crystal Deposition by Regulating TFEB-Induced Autophagy Pathway. Front Cell Dev Biol 9:638759 (2021). PubMed: 33718378
- Liu M et al. Microbial genetic and transcriptional contributions to oxalate degradation by the gut microbiota in health and disease. Elife 10:N/A (2021). PubMed: 33769280
- Zhu J et al. Inhibiting inflammation and modulating oxidative stress in oxalate-induced nephrolithiasis with the Nrf2 activator dimethyl fumarate. Free Radic Biol Med 134:9-22 (2018). PubMed: 30599261
- Li Y et al. Response of Npt2a knockout mice to dietary calcium and phosphorus. PLoS One 12:e0176232 (2017). Mouse . PubMed: 28448530