Oxalate Decarboxylase Activity Assay Kit (Colorimetric) (ab196993)

Overview

  • Product name

    Oxalate Decarboxylase Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Tissue
  • Assay type

    Enzyme activity
  • Species reactivity

    Reacts with: Plants, Fungi
  • Product overview

    Oxalate Decarboxylase Activity Assay Kit (Colorimetric) (ab196993) provides a quick and easy way to measure oxalate decarboxylase (OXDC) activity in a variety of plant and fungi samples. Oxalate is converted by OXDC to formate, which in turn converts a nearly colorless probe to a colored product with strong absorbance at OD=450 nm. The sensitivity of this assay is less than 20 μU of OXDC activity.


    The assay is simple, sensitive, high-throughput adaptable.

  • Notes

    Oxalate Decarboxylase (OXDC, EC 4.1.1.2) belongs to the cupin superfamily and is composed of two β-barrel domains. OXDC catalyzes the conversion of Oxalate into Formate and CO2 and plays an important role in stress response. In humans, high levels of oxalate can lead to various health problems including hyperoxaluria, kidney stones, and renal failure. Wood rotting fungi generates high levels of oxalate, causing rot in many crops including lettuce, soybean, dry bean, and tomato etc. Recent studies show that overexpression of OXDC in plants (e.g. tomato, soybean, lettuce, and tobacco) results in transgenic plants with resistance to fungal pathogenesis. Accurate measurement of Oxalate Decarboxylase activity is useful for a variety of therapeutic, diagnostic, and mechanistic studies.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Formate Standard (100 mM) Yellow cap 1 x 0.1ml
    OXDC Assay Buffer I 1 x 20ml
    OXDC Assay Buffer II 1 x 15ml
    OXDC Enzyme Mix 1 vial
    OXDC Probe 1 vial
    OXDC Substrate 1 vial
    Positive Control 1 x 0.1ml

Images

  • Oxalate Decarboxylase (OXDC) activity was measured in white mushroom lysate (20 μL), and Jade Plant lysate (20 μL).

  • TYpical Formate Standard Curve.

Protocols

References

ab196993 has not yet been referenced specifically in any publications.

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