Overview

  • Product name

    Oxaloacetate Assay Kit
  • Detection method

    Colorimetric/Fluorometric
  • Sample type

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type

    Quantitative
  • Sensitivity

    > 2 µM
  • Range

    2 µM - 200 µM
  • Assay time

    0h 40m
  • Product overview

    Abcam's Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples. In the assay, OAA is converted to pyruvate which is utilized to convert a nearly colorless probe to an intensely colored (?max= 570nm) and fluorescent (Ex/Em = 535/587nm) product. The Oxaloacetate Assay Kit can detect 0.1-10nmol (2-200 µM) of OAA.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Oxaloacetate (OAA, HOOC-CO-CH2-COOH) is a TCA cycle intermediate. It precedes citrate which is formed by the transfer of an acetyl group to OAA. OAA is formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase

  • Platform

    Microplate reader

Properties

Associated products

Images

  • Oxaloacetate fluorimetrically measured in biofluids (after spin column) showing concentration (micromolar)

  • Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted

  • Oxaloacetate standard curve generated following the kit protocol.

Protocols

References

This product has been referenced in:

  • Wang J  et al. miR-9-5p inhibits pancreatic cancer cell proliferation, invasion and glutamine metabolism by targeting GOT1. Biochem Biophys Res Commun 509:241-248 (2019). Read more (PubMed: 30591220) »
  • Park S  et al. High activity and stability of codon-optimized phosphoenolpyruvate carboxylase from Photobacterium profundum SS9 at low temperatures and its application for in vitro production of oxaloacetate. Bioprocess Biosyst Eng 37:331-5 (2014). Read more (PubMed: 23719931) »
See all 4 Publications for this product

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