• Product name

  • Description

    Rabbit polyclonal to OXCT1/SCOT
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human OXCT1/SCOT aa 171-434. (BC009001)

  • Positive control

    • Human fetal heart and brain lysates; Human fetal kidney tissue.
  • General notes

    Protein previously labeled as OXCT1.



Our Abpromise guarantee covers the use of ab105320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
IHC-P 1/100 - 1/500.


  • Function

    Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
  • Tissue specificity

    Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
  • Pathway

    Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.
  • Involvement in disease

    Succinyl-CoA:3-oxoacid CoA transferase deficiency
  • Sequence similarities

    Belongs to the 3-oxoacid CoA-transferase family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 oxoacid CoA transferase 1 antibody
    • 3-oxoacid CoA-transferase 1 antibody
    • EC antibody
    • OTTHUMP00000120012 antibody
    • OTTHUMP00000221550 antibody
    • OXCT antibody
    • Oxct1 antibody
    • SCOT antibody
    • Scot S antibody
    • Scot-S antibody
    • SCOT1_HUMAN antibody
    • Somatic type succinyl CoA:3 oxoacid CoA transferase antibody
    • Somatic-type succinyl-CoA:3-oxoacid CoA-transferase antibody
    • Succinyl CoA:3 ketoacid CoA transferase antibody
    • Succinyl CoA:3 ketoacid coenzyme A transferase 1 mitochondrial antibody
    • Succinyl CoA:3 oxoacid CoA transferase antibody
    • Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial antibody
    see all


  • All lanes : Anti-OXCT1/SCOT antibody (ab105320) at 1/1000 dilution

    Lane 1 : HEK293 whole cell lysate with SEA BLOCK blocking buffer
    Lane 2 : Mitochondria isolated from HEK293 cells with SEA BLOCK blocking buffer
    Lane 3 : Mitochondria isolated from non-synaptosomal human brain tissue. with SEA BLOCK blocking buffer
    Lane 4 : Mitochondria isolated from synaptosomal human neural tissue. with SEA BLOCK blocking buffer
    Lane 5 : Non-synaptosomal murine brain mitochondria (-ve control) with SEA BLOCK blocking buffer
    Lane 6 : Synaptosomal murine neural mitochondria (-ve control) with SEA BLOCK blocking buffer
    Lane 7 : STHdh-Q7/Q7 mitochondria (-ve control) with SEA BLOCK blocking buffer
    Lane 8 : STHdh-Q111/Q111 mitochondria (-ve control) with SEA BLOCK blocking buffer

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 100 % per lane.

    All lanes : Goat anti-rabbit IRDye® 800CW at 1/30000 dilution

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Additional bands at: 20 kDa (possible non-specific binding), 25 kDa (possible non-specific binding), 52 kDa (possible post-translational modification), 70 kDa (possible non-specific binding)

    Exposure time: 5 minutes

    See Abreview

  • All lanes : Anti-OXCT1/SCOT antibody (ab105320) at 1/500 dilution

    Lane 1 : Human fetal heart lysate
    Lane 2 : Human fetal brain lysate

    Predicted band size: 56 kDa

  • ab105320 at 1/100 dilution staining OXCT1/SCOT in Human fetal kidney tissue


This product has been referenced in:

  • Schnyder S  et al. Muscle PGC-1a is required for long-term systemic and local adaptations to a ketogenic diet in mice. Am J Physiol Endocrinol Metab 312:E437-E446 (2017). Read more (PubMed: 28223292) »
  • Svensson K  et al. Skeletal muscle PGC-1a modulates systemic ketone body homeostasis and ameliorates diabetic hyperketonemia in mice. FASEB J 30:1976-86 (2016). Read more (PubMed: 26849960) »
See all 3 Publications for this product

Customer reviews and Q&As

Western blot
Mouse Cell lysate - other (Mice brain, and cell line mitochondria)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Mice brain, and cell line mitochondria
Blocking step
SEA BLOCK Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C

Svitlana Yablonska

Verified customer

Submitted Aug 24 2015

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