Anti-OXCT1/SCOT antibody (ab224250)
Key features and details
- Rabbit polyclonal to OXCT1/SCOT
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-OXCT1/SCOT antibody
See all OXCT1/SCOT primary antibodies -
Description
Rabbit polyclonal to OXCT1/SCOT -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment corresponding to Human OXCT1/SCOT aa 50-250.
Database link: P55809 -
Positive control
- WB: HeLa, NIH/3T3, Jurkat and NBT-II cell lysates; human liver, heart, testis and kidney tissue lysates. IHC-P: Human heart muscle tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 56 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 56 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate. -
Tissue specificity
Abundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts. -
Pathway
Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1. -
Involvement in disease
Succinyl-CoA:3-oxoacid CoA transferase deficiency -
Sequence similarities
Belongs to the 3-oxoacid CoA-transferase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 5019 Human
- Entrez Gene: 67041 Mouse
- Entrez Gene: 396978 Pig
- Entrez Gene: 690163 Rat
- Omim: 601424 Human
- SwissProt: P55809 Human
- SwissProt: Q9D0K2 Mouse
- SwissProt: Q29551 Pig
see all -
Alternative names
- 3 oxoacid CoA transferase 1 antibody
- 3-oxoacid CoA-transferase 1 antibody
- EC 2.8.3.5 antibody
see all
Images
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All lanes : Anti-OXCT1/SCOT antibody (ab224250) at 0.04 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : OXCT1 knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 18 µg per lane.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDaFalse colour image of Western blot: Anti-OXCT1/SCOT antibody staining at 0.04 µg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab224250 was shown to bind specifically to OXCT1/SCOT. A band was observed at 56 kDa in wild-type HeLa cell lysates with no signal observed at this size in OXCT1 knockout cell line ab265358 (knockout cell lysate ab258557). To generate this image, wild-type and OXCT1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Lane 1 : ab224250 at 0.4 µg/ml
Lane 2 : Anti-OXCT1/SCOT antibody (ab224250) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate
Lane 2 : A549 (Human lung carcinoma cell line) lysate
Predicted band size: 56 kDaLoading control: Anti-COX4I1.
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All lanes : Anti-OXCT1/SCOT antibody (ab224250) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) cell lysate
Predicted band size: 56 kDa -
Paraffin-embedded human testis stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cells in seminiferous ducts.
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Paraffin-embedded human heart stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cardiomyocytes.
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Paraffin-embedded human liver stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes as expected.
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Paraffin-embedded human kidney stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cells in tubules.
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PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for OXCT1/SCOT (green) using ab224250 (4 µg/ml) in ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224250 has been referenced in 1 publication.
- Xie J et al. Hyperinsulinemia impairs the metabolic switch to ketone body utilization in proximal renal tubular epithelial cells under energy crisis via the inhibition of the SIRT3/SMCT1 pathway. Front Endocrinol (Lausanne) 13:960835 (2022). PubMed: 36237185