Key features and details
- Rabbit polyclonal to OXCT1/SCOT
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-OXCT1/SCOT antibody
See all OXCT1/SCOT primary antibodies
DescriptionRabbit polyclonal to OXCT1/SCOT
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Pig
- WB: HeLa, NIH/3T3 and NBT-II cell lysates; human liver, heart, testis and kidney tissue lysates. IHC-P: Human heart muscle tissue. ICC/IF: A431 cells.
Protein previously labeled as OXCT1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab224250 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 56 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionKey enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Tissue specificityAbundant in heart, followed in order by kidney, brain, and muscle, whereas in liver it is undetectable; also detectable in leukocytes and fibroblasts.
PathwayKetone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.
Involvement in diseaseSuccinyl-CoA:3-oxoacid CoA transferase deficiency
Sequence similaritiesBelongs to the 3-oxoacid CoA-transferase family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 3 oxoacid CoA transferase 1 antibody
- 3-oxoacid CoA-transferase 1 antibody
- EC 18.104.22.168 antibody
Paraffin-embedded human kidney stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cells in tubules.
Paraffin-embedded human testis stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cells in seminiferous ducts.
Paraffin-embedded human heart stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows strong cytoplasmic positivity in cardiomyocytes.
Paraffin-embedded human liver stained for OXCT1 using ab224250 at 1/200 dilution in immunohistochemical analysis. Shows no positivity in hepatocytes as expected.
Lane 1 : ab224250 at 0.4 µg/ml
Lane 2 : Anti-OXCT1/SCOT antibody (ab224250) at 0.4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate
Lane 2 : A549 (Human lung carcinoma cell line) lysate
Predicted band size: 56 kDa
Loading control: Anti-COX4I1.
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for OXCT1/SCOT (green) using ab224250 (4 µg/ml) in ICC/IF.
All lanes : Anti-OXCT1/SCOT antibody (ab224250) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Lane 2 : NBT-II (rat Wistar bladder tumor cell line) cell lysate
Predicted band size: 56 kDa
ab224250 has not yet been referenced specifically in any publications.