Overview

  • Product name

    Anti-OXGR1/GPR99 antibody [EPR6305(2)]
    See all OXGR1/GPR99 primary antibodies
  • Description

    Rabbit monoclonal [EPR6305(2)] to OXGR1/GPR99
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human OXGR1/GPR99 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • A549, NIH 3T3, fetal brain, fetal kidney and placenta lysates; NIH 3T3 cells.
  • General notes

     

     

     This product was previously labelled as OXGR1

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR6305(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab140630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Receptor for alpha-ketoglutarate. Seems to act exclusively through a G(q)-mediated pathway.
    • Tissue specificity

      Detected in kidney and, to a lower extend, in placenta. Not detected in brain tissues including the frontal cortex, caudate putamen, thalamus, hypothalamus, hippocampus or pons.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2 oxoglutarate receptor 1 antibody
      • 2-oxoglutarate receptor 1 antibody
      • aKGR antibody
      • Alpha ketoglutarate receptor 1 antibody
      • Alpha-ketoglutarate receptor 1 antibody
      • G Protein Coupled Receptor 80 antibody
      • G Protein Coupled Receptor 99 antibody
      • G-protein coupled receptor 80 antibody
      • G-protein coupled receptor 99 antibody
      • GPR80 antibody
      • GPR99 antibody
      • MGC119206 antibody
      • MGC119207 antibody
      • MGC119208 antibody
      • OXGR 1 antibody
      • Oxgr1 antibody
      • OXGR1_HUMAN antibody
      • Oxoglutarate (alpha ketoglutarate) receptor 1 antibody
      • Oxoglutarate receptor 1 antibody
      • P2RY15 antibody
      • P2Y like GPCR antibody
      • P2Y like nucleotide receptor antibody
      • P2Y purinoceptor 15 antibody
      • P2Y-like GPCR antibody
      • P2Y-like nucleotide receptor antibody
      • P2Y15 antibody
      see all

    Images

    • Overlay histogram showing HEK293 cells stained with ab140630 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab140630, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • All lanes : Anti-OXGR1/GPR99 antibody [EPR6305(2)] (ab140630) at 1/1000 dilution

      Lane 1 : A549 cell lysate
      Lane 2 : NIH 3T3 cell lysate
      Lane 3 : Fetal brain tissue lysate
      Lane 4 : Fetal kidney tissue lysate
      Lane 5 : Placenta tissue lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 38 kDa

    • Immunofluorescent staining of NIH 3T3 cells labelling OXGR1/GPR99 with ab140630 at 1/250 dilution.

    References

    This product has been referenced in:

    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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