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Oxidized HDL Assay Kit (CML-HDL, Human) (ab242309)

  • Datasheet
  • SDS
  • Protocol Booklet
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Human CML-HDL Standard Curve
  • CML-HDL Determination of Serum and Plasma Samples.

Key features and details

  • Detection method: Colorimetric
  • Platform: Microplate
  • Sample type: Plasma, Serum

Overview

  • Product name

    Oxidized HDL Assay Kit (CML-HDL, Human)
    See all oxidized phospholipids kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma
  • Species reactivity

    Reacts with: Human
  • Product overview

    Oxidized HDL Assay Kit (CML-HDL, Human) (ab242309) is an enzyme immunoassay developed for the detection and quantitation of human OxHDL in plasma or serum samples.


    The kit contains a CML-HDL standard and has a detection sensitivity limit of <1 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.


     

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 100ml
    5X Wash Solution 1 x 1.2ml
    Anti-CML Antibody Coated Plate 1 unit
    Assay Diluent 1 x 50ml
    Biotinylated Anti-Human ApoAI Antibody (1000X) 1 x 20µl
    Blocking Reagent (100X) 1 x 200µl
    Buffer Solution 1 x 5ml
    CML-HDL Standard 1 x 20µl
    Precipitation Solution 1 1 x 250µl
    Precipitation Solution 2 1 x 2.5ml
    Precipitation Solution 3 1 x 650µl
    Stop Solution 1 x 12ml
    Streptavidin-Enzyme Conjugate 1 x 20µl
    Substrate Solution 1 x 12ml
  • Relevance

    Lipoproteins are submicroscopic particles composed of lipid and protein held together by noncovalent forces. Their general structure is that of a putative spheroidal microemulsion formed from an outer layer of phospholipids, unesterified cholesterol, and proteins, with a core of neutral lipids, predominately cholesteryl esters and triacylglycerols (TAG). Low density lipoprotein (LDL) is the major transport protein for cholesterol in human plasma. LDL is a spherical particle with a diameter of 20-25 nm. Each LDL particle contains cholesteryl esters in its core which are surrounded by a hydrophilic coat composed of phospholipids, cholesterol, and one molecule of a hydrophobic protein known as apolipoprotein B-100. LDL cholesterol, sometimes referred to as “bad” cholesterol, is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. LDL oxidation affects both the protein and phospholipids of LDL. Reactive aldehyde products formed during the oxidation of polyunsaturated fatty acids, such as malondialdehyde (MDA) and 4hydroxynonenal (HNE), are capable of attaching covalently to the e-amino groups of lysine residues of of ApoB-100 to form MDA-Lys and HNE-Lys adducts (MDA-LDL and HNE-LDL). Advanced glycosylation, such as the formation of CML-LDL and CEL-LDL, are also involved in LDL oxidation.

Applications

Our Abpromise guarantee covers the use of ab242309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Human CML-HDL Standard Curve
    Human CML-HDL Standard Curve
  • CML-HDL Determination of Serum and Plasma Samples.
    CML-HDL Determination of Serum and Plasma Samples.

    HDL from human serum and plasma samples was isolated according to the Sample Preparation Section. Samples were diluted from 5 mg/mL to 64 µg/mL in Assay Diluent, and then fourfold serial dilutions were prepared. Samples were tested according to the Assay Protocol. 

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab242309? Please let us know so that we can cite the reference in this datasheet.

    ab242309 has not yet been referenced specifically in any publications.

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