Key features and details
- Detection method: Colorimetric
- Platform: Microplate
- Sensitivity: 150 ng/ml
Product nameOxidized LDL Assay Kit (HNE-LDL, Human)
See all oxidized phospholipids kits
Species reactivityReacts with: Human
The Oxidized LDL Assay Kit (HNE-LDL, Human) ELISA Kit (ab242304) is developed for the detection and quantitation of human oxLDL in plasma, serum or other biological fluid samples.
The kit contains a copper oxidized LDL standard and has a detection sensitivity limit of ~150 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.
Tested applicationsSuitable for: ELISAmore details
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 10X Wash Buffer 1 x 100ml Anti-HNE Antibody Coated Plate 1 unit Assay Diluent 1 x 50ml Biotinylated Anti-Human ApoB-100 Antibody (1000X) 1 x 20µl Blocking Reagent (100X) 1 x 200µl LDL Precipitation Solution (2X) 1 x 20ml OxLDL Standard 1 x 25µl Stop Solution 1 x 12ml Streptavidin-Enzyme Conjugate 1 x 20µl Substrate Solution 1 x 12ml
RelevanceLipoproteins are submicroscopic particles composed of lipid and protein held together by noncovalent forces. Their general structure is that of a putative spheroidal microemulsion formed from an outer layer of phospholipids, unesterified cholesterol, and proteins, with a core of neutral lipids, predominately cholesteryl esters and triacylglycerols (TAG). Low density lipoprotein (LDL) is the major transport protein for cholesterol in human plasma. LDL is a spherical particle with a diameter of 20-25 nm. Each LDL particle contains cholesteryl esters in its core which are surrounded by a hydrophilic coat composed of phospholipids, cholesterol, and one molecule of a hydrophobic protein known as apolipoprotein B-100. LDL cholesterol, sometimes referred to as “bad” cholesterol, is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. LDL oxidation affects both the protein and phospholipids of LDL. Reactive aldehyde products formed during the oxidation of polyunsaturated fatty acids, such as malondialdehyde (MDA) and 4hydroxynonenal (HNE), are capable of attaching covalently to the e-amino groups of lysine residues of of ApoB-100 to form MDA-Lys and HNE-Lys adducts (MDA-LDL and HNE-LDL). Advanced glycosylation, such as the formation of CML-LDL and CEL-LDL, are also involved in LDL oxidation.
Our Abpromise guarantee covers the use of ab242304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
Left: LDL Recovery After Precipitation Solution. Serum and plasma samples were treated with LDL Precipitation Solution according to the Sample Preparation protocol. LDL recovery was determined. Right: OxLDL Determination of Serum and Plasma Samples. Serum and plasma samples were treated with LDL Precipitation Solution according to the Sample Preparation Section. Precipitated LDL pellets were resuspended in 1.6 mL of PBS before further diluting 1:50 in Assay Diluent. Samples were tested according to the Assay Protocol.
ab242304 has not yet been referenced specifically in any publications.