Overview

  • Product name

    Oxidized LDL Assay Kit (MDA-LDL, Human)
    See all oxidized phospholipids kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Plasma
  • Sensitivity

    50 ng/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Oxidized LDL Assay Kit (MDA-LDL, Human) ELISA Kit (ab242302) is developed for the detection and quantitation of oxidized phospholipids (OxPL) associated with human LDL in plasma, serum or other biological fluid samples.


    The kit contains an OxLDL Standard and has a detection sensitivity limit of <15 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and unknown samples.


     


     

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 100ml
    Anti-MDA Antibody Coated Plate 1 unit
    Assay Diluent 1 x 50ml
    Biotinylated Anti-Human ApoB-100 Antibody (1000X) 1 x 20µl
    Blocking Reagent (100X) 1 x 200µl
    LDL Precipitation Solution 1 x 20ml
    OxLDL Standard 1 x 25µl
    Stop Solution 1 x 12ml
    Streptavidin-Enzyme Conjugate 1 x 20µl
    Substrate Solution 1 x 12ml
  • Relevance

    Lipoproteins are submicroscopic particles composed of lipid and protein held together by noncovalent forces. Their general structure is that of a putative spheroidal microemulsion formed from an outer layer of phospholipids, unesterified cholesterol, and proteins, with a core of neutral lipids, predominately cholesteryl esters and triacylglycerols (TAG). Low density lipoprotein (LDL) is the major transport protein for cholesterol in human plasma. LDL is a spherical particle with a diameter of 20-25 nm. Each LDL particle contains cholesteryl esters in its core which are surrounded by a hydrophilic coat composed of phospholipids, cholesterol, and one molecule of a hydrophobic protein known as apolipoprotein B-100. LDL cholesterol, sometimes referred to as “bad” cholesterol, is even more dangerous when it becomes oxidized. Oxidized LDL (OxLDL) is more reactive with surrounding tissues and can collect within the inner-lining of arteries. Macrophages, cholesterol, and other lipids can accumulate at the site (atherosclerosis), ultimately forming a plaque that can lead to heart attack, stroke or death. LDL oxidation affects both the protein and phospholipids of LDL. Reactive aldehyde products formed during the oxidation of polyunsaturated fatty acids, such as malondialdehyde (MDA) and 4hydroxynonenal (HNE), are capable of attaching covalently to the e-amino groups of lysine residues of of ApoB-100 to form MDA-Lys and HNE-Lys adducts (MDA-LDL and HNE-LDL). Advanced glycosylation, such as the formation of CML-LDL and CEL-LDL, are also involved in LDL oxidation.

Applications

Our Abpromise guarantee covers the use of ab242302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Left: LDL Recovery After Precipitation Solution. Serum and plasma samples were treated with LDL Precipitation Solution according to the Sample Preparation protocol. LDL recovery was determined. Right: OxLDL Determination of Serum and Plasma Samples. Serum and plasma samples were treated with LDL Precipitation Solution according to the Sample Preparation Section. Precipitated LDL pellets were resuspended in 1.6 mL of PBS before further diluting 1:160 in Assay Diluent. Samples were tested according to the Assay Protocol.

Protocols

References

ab242302 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab242302.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up