Overview

  • Product name

    Anti-Oxoguanine 8 antibody [2Q2311]
  • Description

    Mouse monoclonal [2Q2311] to Oxoguanine 8
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, IHC, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type. Oxoguanine 8 adsorbed on to alumina.

  • Positive control

    • ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab206461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC 1/50.
ICC/IF 1/50.

Target

  • Relevance

    Oxoguanine 8 (8-Oxoguanine) is a mutagenic oxidative damage product of guanine. Guanine is the main target for reactive oxygen species in DNA, with 8-oxoguanine being the most frequent base lesion. Thus formation of 8-oxoguanine is an important biomarker of oxidative damage to DNA. It is primarily repaired by the DNA glycosylase OGG1.
  • Cellular localization

    Nuclear
  • Alternative names

    • 8 Oxoguanine antibody
    • 8 hydroxyguanine antibody
    • 8 Oxo 7,8 dihydroguanine antibody
    • 8 oxo Gua antibody
    • 8 oxoG antibody
    see all

Images

  • Immunofluorescent staining of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells using ab206461 at 1/50. TRITC-conjugated goat anti-mouse IgG was used as the secondary. On the left, the nuclei were stained with DAPI and the actin filaments with an Alexa Fluor® 488 conjugated antibody. On the right, the ab206461 staining is shown (red).

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
Yes - Citrate buffer
Specification
heart
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 29 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up