Overview

  • Product name

    Oxonol V, Fluorescent membrane indicator
  • Description

    Anionic dye. Fluorescent membrane potential indicator.
  • Biological description

    Anionic dye. Fluorescent membrane potential indicator. Carries a negative electric charge. Monitors positive membrane potentials inside vesicles. Provides estimates of transmembrane potentials.
  • Purity

    > 95%
  • CAS Number

    61389-30-8
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    560nm
  • Emission

    560nm
  • Chemical name

    1,5-Bis(3-phenyl-5-oxoisoxazol-4-yl)pentamethine oxonol
  • Molecular weight

    384.38
  • Molecular formula

    C23H16N2O4
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in ethanol and in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    Oc4onc(c1ccccc1)c4/C=C/C=C/C=C3\C(=O)ON=C3c2ccccc2
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Holoubek A  et al. Ratiometric fluorescence measurements of membrane potential generated by yeast plasma membrane H(+)-ATPase reconstituted into vesicles. Biochim Biophys Acta 1609:71-9 (2003). Read more (PubMed: 12507760) »
  • Cooper CE  et al. Use of oxonol V as a probe of membrane potential in proteoliposomes containing cytochrome oxidase in the submitochondrial orientation. Biochemistry 29:3859-65 (1990). Read more (PubMed: 2162199) »
  • Scherman D & Henry JP Oxonol-V as a probe of chromaffin granule membrane potentials. Biochim Biophys Acta 599:150-66 (1980). Read more (PubMed: 7397145) »

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