Key features and details
- Rabbit polyclonal to OXSR1 (phospho T185)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-OXSR1 (phospho T185) antibody
See all OXSR1 primary antibodies
DescriptionRabbit polyclonal to OXSR1 (phospho T185)
Specificityab192803 detects endogenous levels of OXSR1 only when phosphorylated at Threonine 185.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human OXSR1 (phospho T185) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of threonine185(R-K-T(p)-F-V).
Database link: O95747
- HepG2 cell extract; Human breast carcinoma tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab192803 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab192803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 58 kDa.|
|IHC-P||1/50 - 1/100.|
FunctionRegulates downstream kinases in response to environmental stress. May also have a function in regulating the actin cytoskeleton.
Tissue specificityUbiquitously expressed in all tissue examined.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- 2210022N24Rik antibody
- 2810422B09Rik antibody
- AI462649 antibody
All lanes : Anti-OXSR1 (phospho T185) antibody (ab192803) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with antigen-specific peptide
Predicted band size: 58 kDa
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling OXSR1 (phospho T185) with ab192803 at 1/50 (left) or ab192803 preincubated with blocking peptide (right).
ab192803 has not yet been referenced specifically in any publications.