Recombinant
RabMAb

Recombinant Anti-Oxytocin antibody [EPR20973] - BSA and Azide free (ab226490)

Overview

  • Product name

    Anti-Oxytocin antibody [EPR20973] - BSA and Azide free
    See all Oxytocin primary antibodies
  • Description

    Rabbit monoclonal [EPR20973] to Oxytocin - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human Oxytocin aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P01178

  • Positive control

    • IHC-P: Mouse hypothalamus tissue.
  • General notes

    Ab226490 is the carrier-free version of ab212193. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab226490 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab226490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat brain (paraventricular nucleus) tissue labeling Oxytocin with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on rat brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212193).

  • Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse brain (paraventricular nucleus) tissue labeling Oxytocin with ab212193 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Cytoplasmic staining on neurons located at the paraventricular nucleus (PVN) on mouse brain tissue section (PMID:24464043). The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212193).

  • Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labeling Oxytocin with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of rat paraventricular nucleus and supraoptic nucleus (PMID: 24464043). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212193).

  • Immunohistochemical analysis of paraffin-embedded mouse hypothalamus tissue labeling Oxytocin with ab212193 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on the neurons of mouse paraventricular nucleus (PMID: 24464043). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212193).

References

ab226490 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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