Product nameOxytocin ELISA Kit
See all Oxytocin kits
Intra-assay Sample n Mean SD CV% Low 20 39.9pg/ml 12.6% Medium 20 121.4pg/ml 10.2% High 20 363.7pg/ml 13.3% Inter-assay Sample n Mean SD CV% Low 17 47pg/ml 20.9% Medium 17 145.1pg/ml 16.5% High 17 397.2pg/ml 11.8%
Sample typeCell culture supernatant, Milk, Urine, Serum, Plasma
Range15.6 pg/ml - 1000 pg/ml
Sample specific recovery Sample type Average % Range Saliva 90 % - % Urine 111 % - % Serum 104 % - %
Assay time25h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Predicted to work with: a wide range of other species
Abcam’s Oxytocin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Oxytocin in Tissue Culture media, Human Serum, Saliva, Cerebrospinal Fluid and Urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Oxytocin antigen and a polyclonal rabbit antibody specific to Oxytocin. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Oxytocin captured in the plate.
Oxytocin is a neurohypophysial peptide which is produced in the paraventricular nuclei of the hypothalamus and stored in the posterior pituitary. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. A hormone once thought to be limited to female smooth muscle reproductive physiology, more current findings have determined that oxytocin also functions as a neurotransmitter, may be involved in neuropsychiatric disorders, social/sexual behavior and is important in male reproductive physiology. Oxytocin and the related neurohypophysial peptide, Arg-Vasopressin, maintain renal water and sodium balance.
Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the Oxytocin-like peptide, mesotocin, a common peptide found in some fishes, reptiles, amphibians, marsupials and nonmammalian tetrapods, the leucine at residue 8 is substituted for isoleucine. Acting in classical endocrine fashion, Oxytocin elicits regulatory effects by binding specific cell surface receptors which in turn initiate a secondary intracellular response cascade via a phosphoinositide signaling pathway.
Compound % Cross Reactivity Mesotocin 7.0 Oxytocin 100 Arg8-Vasotocin 7.5 Ser4,Ile8-Oxytocin <0.2 TRH <0.2 Somatostatin <0.2 Met-Enkephalin <0.2 VIP <0.2 Lys8-Vasopressin <0.2 Arg8-Vasopressin <0.2 α−ANP <0.2 Growth Hormone <0.2 Tocinoic acid <0.2 Melanostatin <0.2
Tested applicationsSuitable for: Competitive ELISAmore details
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Oxytocin Alkaline Phosphatase Conjugate 1 x 5ml Oxytocin Antibody 1 x 5ml Oxytocin Standard 1 x 0.5ml Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml
- OT NPI
Our Abpromise guarantee covers the use of ab133050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent concentration.|
This product has been referenced in:
- Dayanithi G et al. Vasopressin and oxytocin in sensory neurones: expression, exocytotic release and regulation by lactation. Sci Rep 8:13084 (2018). Read more (PubMed: 30166555) »
- Bomann AC et al. The neurobiology of social deficits in female patients with borderline personality disorder: The importance of oxytocin. Personal Ment Health 11:91-100 (2017). Human . Read more (PubMed: 28397403) »