Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
hydrogen peroxide as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate, pH6.0, 100C 20 min
Sample
Human Tissue sections (placenta)
Specification
placenta
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent, background reducers
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Leica Refine
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
various retrieval modalities tested citrate gave best reaction to background
Abcam user community
Verified customer
Submitted Jul 01 2014