• Product name

    Anti-Oxytocin-neurophysin 1 antibody
    See all Oxytocin-neurophysin 1 primary antibodies
  • Description

    Rabbit polyclonal to Oxytocin-neurophysin 1
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Horse, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Oxytocin-neurophysin 1 aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: P01178

  • Positive control

    • This antibody gave a positive signal in Human adult pituitary gland tissue lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa.



Our Abpromise guarantee covers the use of ab67457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12, 10 kDa (predicted molecular weight: 12 kDa).
ICC/IF Use a concentration of 5 µg/ml.


  • Relevance

    Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Oxytocin contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.
  • Cellular localization

  • Database links

  • Alternative names

    • MGC126890 antibody
    • MGC126892 antibody
    • Neurophysin 1 antibody
    • Neurophysin I antibody
    • Ocytocin antibody
    • OT antibody
    • OT NPI antibody
    • OXT antibody
    • Oxytocin Neurophysin preproprotein antibody
    • Oxytocin prepro (neurophysin I) antibody
    • Oxytocin prepropeptide antibody
    • Oxytocin-neurophysin 1 antibody
    see all


  • Anti-Oxytocin-neurophysin 1 antibody (ab67457) at 1 µg/ml + Brain: pituitary gland (Human) Whole Cell Lysate - adult normal tissue (ab29749) at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilution (Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) )

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa
    Additional bands at: 10 kDa (possible cleavage fragment)

    We hypothesize that the 12 and 10 kDa bands represent the pro and mature form of Oxytocin, respectively.
  • ICC/IF image of ab67457 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab67457 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


ab67457 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you for your inquiry.

You are correct that the mature Oxytoxin (CYIQNCPLG)is a nonapeptide and too short to analyse on a WB.

I can confirm that ab67457 recognises the prepropeptide of oxytoxin, also known as Oxytocin-neurophysin 1 (OT-NPI).

More information about this protein can be found following this link to the Swiss Prot homepage:


This antibody is only tested for WB and we do not know yet if it is suitable for IHC.

We do offer two antibodiesthat are tested and guaranteed for IHC-P and one for rat and IHC-Fr. Please follow these links to review the respective datasheets:

https://www.abcam.com/index.html?datasheet=2078 (or use the following: https://www.abcam.com/index.html?datasheet=2078).

https://www.abcam.com/index.html?datasheet=78364 (or use the following: https://www.abcam.com/index.html?datasheet=78364).

https://www.abcam.com/index.html?datasheet=11143 (or use the following: https://www.abcam.com/index.html?datasheet=11143).

I hope this information is helpful. Please do not hesitate to contactme again with any further questions in this matter.

Read More

For licensing inquiries, please contact partnerships@abcam.com

Sign up