Recombinant
RabMAb

Recombinant Anti-Oxytocin Receptor antibody [EPR12789] (ab181077)

Overview

  • Product name

    Anti-Oxytocin Receptor antibody [EPR12789]
    See all Oxytocin Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR12789] to Oxytocin Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Oxytocin Receptor aa 50-150. The exact sequence is proprietary.
    Database link: P30559

  • Positive control

    • WB: HeLa, MCF7, Jurkat and PC-3 cell lysates and mouse and rat spleen tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

Target

Images

  • All lanes : Anti-Oxytocin Receptor antibody [EPR12789] (ab181077) at 1/5000 dilution (purified)

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 43 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Oxytocin Receptor antibody [EPR12789] (ab181077) at 1/5000 dilution (purified)

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : PC-3 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 43 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Oxytocin Receptor antibody [EPR12789] (ab181077) at 1/20000 dilution (unpurified)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : PC-3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

References

This product has been referenced in:

  • Yuan W  et al. Role of oxytocin in the medial preoptic area (MPOA) in the modulation of paternal behavior in mandarin voles. Horm Behav 110:46-55 (2019). Read more (PubMed: 30836063) »
  • Hua S & Vaughan B In vitro comparison of liposomal drug delivery systems targeting the oxytocin receptor: a potential novel treatment for obstetric complications. Int J Nanomedicine 14:2191-2206 (2019). Read more (PubMed: 30988616) »
See all 9 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK cells)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
40 µg
Specification
HEK cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2018

Application
Flow Cytometry
Sample
Human Cell (Immortalized human myometrial cells)
Permeabilization
No
Gating Strategy
forward scatter vs. side scatter and live/dead cell staining with cytox red
Specification
Immortalized human myometrial cells
Preparation
Cell harvesting/tissue preparation method: Live cells lifted from flasks using cell dissociation buffer
Sample buffer: PBS with 0.2%BSA and 0.09% sodium azide
Fixation
no fixation

Abcam user community

Verified customer

Submitted Sep 08 2017

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (Bis-Tris gel (10%))
Sample
Human Cell lysate - whole cell (primary myometrial cells)
Specification
primary myometrial cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 14 2014

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