p-Azidophenacyl bromide, photolabile cross-linking reagent (ab144922)

Overview

  • Product name

    p-Azidophenacyl bromide, photolabile cross-linking reagent
  • Description

    Versatile photolabile bifunctional reagent.
  • Alternative names

    • 1-(4-Azidophenyl)-2-bromo-ethanone
    • 4-Azidophenacyl bromide
  • Biological description

    Versatile photolabile bifunctional reagent. Useful for investigating the active sites of sulfhydryl enzymes, particularly those which posess a reactive cysteine residue at the active site.

  • CAS Number

    57018-46-9
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    4′-Azido-2-bromoacetophenone
  • Molecular weight

    240.06
  • Molecular formula

    C8H6BrN3O
  • Storage instructions

    Store at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

This product has been referenced in:

  • Rausch JW  et al. Probing contacts between the ribonuclease H domain of HIV-1 reverse transcriptase and nucleic acid by site-specific photocross-linking. J Biol Chem 275:16015-22 (2000). Read more (PubMed: 10748161) »
  • Soubannier V  et al. The second stalk of the yeast ATP synthase complex: identification of subunits showing cross-links with known positions of subunit 4 (subunit b). Biochemistry 38:15017-24 (1999). Read more (PubMed: 10555984) »
  • Hixson SH & Hixson SS P-Azidophenacyl bromide, a versatile photolabile bifunctional reagent. Reaction with glyceraldehyde-3-phosphate dehydrogenase. Biochemistry 14:4251-4 (1975). Read more (PubMed: 1237309) »

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