Overview

  • Product name

  • Description

    Rabbit polyclonal to P cadherin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 55-289 of Human P cadherin.

  • Positive control

    • 293T whole cell lysate; Human FaDu xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 91 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.
  • Tissue specificity

    Expressed in some normal epithelial tissues and in some carcinoma cell lines.
  • Involvement in disease

    Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
    Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly.
  • Sequence similarities

    Contains 5 cadherin domains.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CADH3_HUMAN antibody
    • Cadherin 3 antibody
    • Cadherin 3 precursor antibody
    • Cadherin 3 type 1 antibody
    • Cadherin-3 antibody
    • Cadp antibody
    • Calcium dependent adhesion protein placental antibody
    • CDH 3 antibody
    • CDH3 antibody
    • CDH3 protein antibody
    • CDHP antibody
    • HJMD antibody
    • P cadherin (placental) antibody
    • P-cadherin antibody
    • PCAD antibody
    • Placental cadherin antibody
    see all

Images

  • Anti-P cadherin antibody (ab137729) at 1/5000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 91 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded FaDu xenograft tissue labelling P cadherin with ab137729 at 1/100 dilution.

References

ab137729 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (mammary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate ph 6,0
Permeabilization
No
Specification
mammary gland
Blocking step
Background Block (CELL MARQUE) as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde

Prof. ALESSANDRA MEDEIROS

Verified customer

Submitted Oct 15 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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