Product nameAnti-P Glycoprotein antibody
See all P Glycoprotein primary antibodies
DescriptionRabbit polyclonal to P Glycoprotein
Tested applicationsSuitable for: WB, IP, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide corresponding to a region within amino acids 1-50 of Human P Glycoprotein (NP_000918.2)
- HeLa, Jurkat and 293T whole cell lysate
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 141 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Tissue specificityExpressed in liver, kidney, small intestine and brain.
Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Information by UniProt
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
All lanes : Anti-P Glycoprotein antibody (ab129450) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 141 kDa
Exposure time: 10 seconds
ICC/IF image of ab129450 stained human hepatocyte-like cells. The cells were fixed in methanol and then incubated in 10% BSA for 1 hour to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab129450, 1/200) overnight at +4°C. The secondary antibody (red) was Alexa Fluor® 568 goat anti-rabbit used at a 1/400 dilution.
Detection of P Glycoprotein by Western Blot of Immunprecipitate.
Lane 1: ab129450 at 1µg/ml staining P Glycoprotein in HeLa whole cell lysate immunoprecipitated using ab129450 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
This product has been referenced in:
- Zhang WY et al. Suppression of long noncoding RNA NCK1-AS1 increases chemosensitivity to cisplatin in cervical cancer. J Cell Physiol 234:4302-4313 (2019). Read more (PubMed: 30221354) »
- Puech C et al. Assessment of HBEC-5i endothelial cell line cultivated in astrocyte conditioned medium as a human blood-brain barrier model for ABC drug transport studies. Int J Pharm 551:281-289 (2018). Read more (PubMed: 30240829) »