Overview

  • Product name

    Anti-P Glycoprotein antibody
    See all P Glycoprotein primary antibodies
  • Description

    Rabbit polyclonal to P Glycoprotein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 1-50 of Human P Glycoprotein (NP_000918.2)

  • Positive control

    • HeLa, Jurkat and 293T whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab129450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 141 kDa.
IP Use at 2-10 µg/mg of lysate.
ICC/IF 1/200.

Target

  • Function

    Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • Tissue specificity

    Expressed in liver, kidney, small intestine and brain.
  • Involvement in disease

    Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABC20 antibody
    • ABCB1 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
    • ATP-binding cassette sub-family B member 1 antibody
    • CD243 antibody
    • CLCS antibody
    • Colchicin sensitivity antibody
    • Doxorubicin resistance antibody
    • GP170 antibody
    • MDR1 antibody
    • MDR1_HUMAN antibody
    • Multidrug resistance 1 antibody
    • Multidrug resistance protein 1 antibody
    • P glycoprotein 1 antibody
    • P gp antibody
    • P-glycoprotein 1 antibody
    • PGY1 antibody
    see all

Images

  • All lanes : Anti-P Glycoprotein antibody (ab129450) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 141 kDa


    Exposure time: 10 seconds
  • ICC/IF image of ab129450 stained human hepatocyte-like cells. The cells were fixed in methanol and then incubated in 10% BSA for 1 hour to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab129450, 1/200) overnight at +4°C. The secondary antibody (red) was Alexa Fluor® 568 goat anti-rabbit used at a 1/400 dilution.

    See Abreview

  • Detection of P Glycoprotein by Western Blot of Immunprecipitate.
    Lane 1: ab129450 at 1µg/ml staining P Glycoprotein in HeLa whole cell lysate immunoprecipitated using ab129450 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:

  • Zhang WY  et al. Suppression of long noncoding RNA NCK1-AS1 increases chemosensitivity to cisplatin in cervical cancer. J Cell Physiol 234:4302-4313 (2019). Read more (PubMed: 30221354) »
  • Puech C  et al. Assessment of HBEC-5i endothelial cell line cultivated in astrocyte conditioned medium as a human blood-brain barrier model for ABC drug transport studies. Int J Pharm 551:281-289 (2018). Read more (PubMed: 30240829) »
See all 23 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for passing on that reply.

I am sorry to hear that the customer has still not observed the expected signal with the anti-P Glycoprotein antibody (ab3366).

As I mentioned in my previous email, I would be happy to provide the customer with a different antibody to try. I would suggest that they have a look at the rabbit polyclonal ab129450 or the mouse monoclonal ab80594. Both of these have been used previously and shown to detect the P Glycoprotein in Western blotting.

The use of the mouse monoclonal ab80594 has been described in the following references:

Chu TM et al. Detection of soluble P-glycoprotein in culture media and extracellular fluids. Biochem Biophys Res Commun 203:506-12 (1994). PubMed: 7915520
Chu TM et al. Characterization of a new monoclonal antibody F4 detecting cell surface epitope and P-glycoprotein in drug-resistant human tumor cell lines. Hybridoma 12:417-29 (1993). PubMed: 7503940



Whilst the results observed with the rabbit polyclonal ab129450 can be observed on the datasheet of the antibody:

https://www.abcam.com/ab129450

Although the Western blot for ab129450 is picking up some background, the band of interest is observed strongly and it may well be that with further optimisation of the blocking conditions etc. that the blot would look much cleaner.

Both of these antibodies would again be covered by the Abpromise guarantee were the customer to have any further problems.

I look forward to receiving your reply.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human liver)
Permeabilization
No
Specification
human liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
methanol:acetone (ratio 1:1)

Abcam user community

Verified customer

Submitted Oct 30 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (hepatocyte-like cells)
Specification
hepatocyte-like cells
Fixative
Methanol
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 13 2013

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