Key features and details
- Rabbit polyclonal to P Glycoprotein
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P Glycoprotein antibody
See all P Glycoprotein primary antibodies
DescriptionRabbit polyclonal to P Glycoprotein
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide corresponding to a region within amino acids 1-50 of Human P Glycoprotein (NP_000918.2)
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 141 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Tissue specificityExpressed in liver, kidney, small intestine and brain.
Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Information by UniProt
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
All lanes : Anti-P Glycoprotein antibody (ab129450) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 141 kDa
Exposure time: 10 seconds
Detection of P Glycoprotein by Western Blot of Immunprecipitate.
Lane 1: ab129450 at 1µg/ml staining P Glycoprotein in HeLa whole cell lysate immunoprecipitated using ab129450 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
ab129450 has been referenced in 35 publications.
- Fan Y et al. Recombinant Dual-target MDM2/MDMX Inhibitor Reverses Doxorubicin Resistance through Activation of the TAB1/TAK1/p38 MAPK Pathway in Wild-type p53 Multidrug-resistant Breast Cancer Cells. J Cancer 11:25-40 (2020). PubMed: 31892970
- Aldonza MBD et al. Prior acquired resistance to paclitaxel relays diverse EGFR-targeted therapy persistence mechanisms. Sci Adv 6:eaav7416 (2020). PubMed: 32083171
- Feng Z & Chen Q Raised CD40L expression attenuates drug resistance in Adriamycin-resistant THP-1 cells. Exp Ther Med 19:2188-2194 (2020). PubMed: 32104283
- Gao J et al. Inhibition of ZEB1-AS1 confers cisplatin sensitivity in breast cancer by promoting microRNA-129-5p-dependent ZEB1 downregulation. Cancer Cell Int 20:90 (2020). PubMed: 32210737
- Kumar P et al. Hsp90 facilitates acquired drug resistance of tumor cells through cholesterol modulation however independent of tumor progression. Biochim Biophys Acta Mol Cell Res 1867:118728 (2020). PubMed: 32343987