Key features and details
- Rabbit polyclonal to P Glycoprotein
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-P Glycoprotein antibody
See all P Glycoprotein primary antibodies
DescriptionRabbit polyclonal to P Glycoprotein
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chinese hamster
- WB: HepG2 and EC109 whole cell lysate. IHC-P: Human liver cancer and placenta tissue. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab235954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000.|
|ICC/IF||1/50 - 1/200.|
FunctionEnergy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
Tissue specificityExpressed in liver, kidney, small intestine and brain.
Involvement in diseaseGenetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
Sequence similaritiesBelongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Contains 2 ABC transmembrane type-1 domains.
Contains 2 ABC transporter domains.
- Information by UniProt
- ABC20 antibody
- ABCB1 antibody
- ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
All lanes : Anti-P Glycoprotein antibody (ab235954) at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : EC109 whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for P Glycoprotein using ab235954 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human placenta tissue stained for P Glycoprotein with ab235954 at a 1/50 dilution in immunohistochemical analysis.
Paraffin-embedded human liver cancer tissue stained for P Glycoprotein with ab235954 at a 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235954 has been referenced in 2 publications.
- Meng D et al. MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells. Onco Targets Ther 11:7733-7743 (2018). PubMed: 30464522
- Li J et al. MicroRNA-140-3p enhances the sensitivity of hepatocellular carcinoma cells to sorafenib by targeting pregnenolone X receptor. Onco Targets Ther 11:5885-5894 (2018). PubMed: 30271172