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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

Reviews (10) Submit a question References (130)
Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
  • Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10364-57] to P Glycoprotein
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-P Glycoprotein antibody [EPR10364-57]
    See all P Glycoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPR10364-57] to P Glycoprotein

  • Host species

    Rabbit

  • Specificity

    P-glycoprotein 1 (also known as Multidrug resistance protein 1) has a predicted molecular weight of 141 kDa, however it has 3 potential glycosylation sites (N-linked) which may affect the migration of the protein. In our hands ab170904 detects a predominant protein band migrating in the region of 180-200 kDa and typically will demonstrate a smear on the membrane in the region of the 150 – 300 kDa due to the glycosylation profile of the protein. It may be necessary to optimise your cell or tissue lysis protocol to efficiently extract P-glycoprotein 1 as it is a multi-pass membrane protein. Abcam recommends not boiling the sample after lysis. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human P Glycoprotein aa 350-750. The exact sequence is proprietary.
    Database link: P08183

  • Positive control

    • WB: HEK-293T, C6, HeLa, HepG2 and HEK-293T cell lysates; human fetal brain tissue lysate; mouse and rat brain and kidney tissue lysates. Wild-type HAP1 cell lysate; IHC-P: Human hepatocellular carcinoma, brain and kidney tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/1200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/20 - 1/100

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat
WB (4)
1/1000 - 1/5000. Predicted molecular weight: 141 kDa.

For optimal detection Abcam recommends not boiling the sample after lysis.
Notes
IHC-P
1/1200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/20 - 1/100

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat
WB
1/1000 - 1/5000. Predicted molecular weight: 141 kDa.

For optimal detection Abcam recommends not boiling the sample after lysis.
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

  • Tissue specificity

    Expressed in liver, kidney, small intestine and brain.

  • Involvement in disease

    Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.

  • Sequence similarities

    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ABC20 antibody
    • ABCB1 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
    • ATP-binding cassette sub-family B member 1 antibody
    • CD243 antibody
    • CLCS antibody
    • Colchicin sensitivity antibody
    • Doxorubicin resistance antibody
    • GP170 antibody
    • MDR1 antibody
    • MDR1_HUMAN antibody
    • Multidrug resistance 1 antibody
    • Multidrug resistance protein 1 antibody
    • P glycoprotein 1 antibody
    • P gp antibody
    • P-glycoprotein 1 antibody
    • PGY1 antibody
    see all

Images

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: P Glycoprotein knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab170904 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab170904 was shown to specifically react with P Glycoprotein when P Glycoprotein knockout samples were used. Wild-type and P Glycoprotein knockout samples were subjected to SDS-PAGE. ab170904 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively.

    Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 0.1 µg/ml (purified)

    Lanes 1 & 3 : C6 (Rat glial tumor glial cell) whole cell lysates prepared using RIPA lysis method
    Lanes 2 & 4 : C6 (Rat glial tumor glial cell) whole cell lysates prepared using 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 141 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab170904 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

    PBS was used instead of the primary antibody as the negative control (inset).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab170904 at 1:1200 dilution (0.24 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Hematoxylinwas used as a counterstain.

    Negative control: PBS instead of the primary antibody (inset).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution (Purified)

    Lanes 1 & 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates prepared using RIPA lysis method
    Lanes 2 & 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate prepared using 1% SDS hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 141 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: Left image -10 seconds; Right image - 1 minute.

    We suggest not to boil the sample after lysis.

    For 1% SDS Hot Lysates preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution (Purified)

    Lanes 1 & 3 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates prepared using RIPA lysis method
    Lanes 2 & 4 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate prepared using 1%SDS lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 141 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST

    Exposure time: Left image - 3 seconds; Right image - 10 seconds.

    We suggest not to boil the sample after lysis.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 4 : Human fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
    Lane 4 : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 141 kDa

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (purified)

    Lane 1 : Mouse brain lysate
    Lane 2 : C6 (Rat glial tumor cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 141 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/2000 dilution (purified)

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 141 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/200 dilution (unpurified)

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 141 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (purified)

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 141 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (unpurified)

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Rat kidney tissue lysate
    Lane 4 : Rat spleen tissue lysate

    Predicted band size: 141 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Western blot - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    All lanes : Anti-P Glycoprotein antibody [EPR10364-57] (ab170904) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 141 kDa
    Observed band size: 180 kDa why is the actual band size different from the predicted?


    Exposure time: 2 minutes
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with unpurified ab170904 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.

    Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with purified ab170904 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.

    Counterstained with hematoxylin.

  • Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)
    Anti-P Glycoprotein antibody [EPR10364-57] (ab170904)

References (130)

Publishing research using ab170904? Please let us know so that we can cite the reference in this datasheet.

ab170904 has been referenced in 130 publications.

  • Feng Y  et al. FOXK1 plays an oncogenic role in the progression of hilar cholangiocarcinoma. Mol Med Rep 23:N/A (2021). PubMed: 33300075
  • Semaan L  et al. MicroRNA-214 enriched exosomes from human cerebral endothelial cells (hCEC) sensitize hepatocellular carcinoma to anti-cancer drugs. Oncotarget 12:185-198 (2021). PubMed: 33613846
  • Chen Y  et al. Inhibition of SENP2-mediated Akt deSUMOylation promotes cardiac regeneration via activating Akt pathway. Clin Sci (Lond) 135:811-828 (2021). PubMed: 33687053
  • Liu Y  et al. Shc3 promotes hepatocellular carcinoma stemness and drug resistance by interacting with ß-catenin to inhibit its ubiquitin degradation pathway. Cell Death Dis 12:278 (2021). PubMed: 33723262
  • Lei G  et al. TRIM29 Reverses Oxaliplatin Resistance of P53 Mutant Colon Cancer Cell. Can J Gastroenterol Hepatol 2021:8870907 (2021). PubMed: 33824865
View all Publications for this product

Customer reviews and Q&As

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1-10 of 10 Abreviews

Immunocytochemistry/ Immunofluorescence abreview for Anti-P Glycoprotein antibody [EPR10364-57]

 Average
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (organoid)
Permeabilization
Yes - 0.5%tritonX-100
Specification
organoid
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 26 2021

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-P Glycoprotein antibody [EPR10364-57]

 Average
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Other
Permeabilization
Yes - triton 1%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Francoise Geffroy

Verified customer

Submitted Feb 19 2020

Western blot abreview for Anti-P Glycoprotein antibody [EPR10364-57]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Pig Cell lysate - whole cell (Brain Endothelial Cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
20 µg
Specification
Brain Endothelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Chloe Whitehouse

Verified customer

Submitted Mar 11 2019

Western blot abreview for Anti-P Glycoprotein antibody [EPR10364-57]

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231 cell lysate, not boiled)
Gel Running Conditions
Reduced Denaturing (4-20% TG gels)
Loading amount
40 µg
Specification
MDA-MB-231 cell lysate, not boiled
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 10 2018

Western blot abreview for Anti-P Glycoprotein antibody [EPR10364-57]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (B16 tumor lysate)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel)
Loading amount
50 µg
Specification
B16 tumor lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 10 2018

Immunohistochemistry (Frozen sections) abreview for Anti-P Glycoprotein antibody [EPR10364-57]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 12% · Temperature: RT°C
Fixative
Methanol followed by Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 15 2017

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-P Glycoprotein antibody [EPR10364-57]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
Other
Permeabilization
Yes - triton 1%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Francoise Geffroy

Verified customer

Submitted Jul 06 2016

Western blot abreview for Anti-P Glycoprotein antibody [EPR10364-57]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast Cancer Cells Adr)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Breast Cancer Cells Adr
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 05 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MDR1 antibody [EPR10364-57]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
peroxidase as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample
Dog Tissue sections (Kidney)
Specification
Kidney
Permeabilization
No
Fixative
Formalin
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 08 2014

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MDR1 [EPR10364] antibody

 Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
None
Sample
Rat Tissue sections (brain)
Specification
brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Kelly Demars

Verified customer

Submitted Jun 24 2013

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