Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MDR1 [EPR10364] antibody

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
None
Sample
Rat Tissue sections (brain)
Specification
brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS
Dilution
1/500

Secondary antibody

Name
Non-Abcam antibody was used: Alexa 488-conjugated AffiniPure Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
ab170904 lot conc. .253mg/ml alexa fluor 488 conc. 0.75 mg/ml

Kelly Demars

Verified customer

Submitted Jun 24 2013

Sign up