Western blot abreview for Anti-P Glycoprotein antibody [EPR10364-57]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast Cancer Cells Adr)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Breast Cancer Cells Adr
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: Cell Signaling secondary antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 140-150 kDa

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Submitted Jun 05 2015

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