Immunohistochemistry (Frozen sections) abreview for Anti-P Glycoprotein antibody [EPR10364-57]

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 12% · Temperature: RT°C
Fixative
Methanol followed by Acetone

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: PBS
Dilution
1/50

Secondary antibody

Dilution
1/50
Name
Non-Abcam antibody was used: Dianova 111-155-003: Ziege IgG anti-Kaninchen IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Aminomethylcoumarin Acetate

Additional data

Additional Notes
A) Immunohistochemical staining of cryosection (IHC-Fr) embedded mouse brain with purified ab170904 at a working dilution of 1:50. The secondary antibody used is goat anti-rabbit IgG H&L at a working dilution of 1:50. B) Counterstaining of PGP (blue, described above) with IgG (red therefore, pink indicates the overlay of PGP and IgG).

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Submitted Mar 15 2017

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