Western blot abreview for Anti-P Glycoprotein antibody [EPR10364-57]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (B16 tumor lysate)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel)
Loading amount
50 µg
Specification
B16 tumor lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IGG HRP 7074 cell signaling
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL with licor c-digit
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 240 kDa

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Submitted Apr 10 2018

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