Western blot abreview for Anti-P Glycoprotein antibody [EPR10364-57]

Poor
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231 cell lysate, not boiled)
Gel Running Conditions
Reduced Denaturing (4-20% TG gels)
Loading amount
40 µg
Specification
MDA-MB-231 cell lysate, not boiled
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Diluent: 5% milk in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IGG HRP 7074 cell signaling
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL with licor c-digit
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 40 kDa Non-specific: 40 kDa

Additional data

Additional Notes
Tried with several human cell lines (OV90, MDA-MB-468), got same results. Worked with a mice cell line (B16)
Abcam response
Thank you for submitting your feedback. These cell types may require treatment for strong expression of P Glycoprotein. you may wish to try a human positive control, such as HepG2 cells.

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Submitted Apr 10 2018

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