Western blot abreview for Anti-P Glycoprotein antibody [EPR10364-57]

Excellent
Abreviews
Application
Western blot
Sample
Pig Cell lysate - whole cell (Brain Endothelial Cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
20 µg
Specification
Brain Endothelial Cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBST
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: sigma a9169 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL Prime
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 140 kDa
Positive control
Pig liver homogenate

Ms. Chloe Whitehouse

Verified customer

Submitted Mar 11 2019

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