Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

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Overview

  • Product name

    Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free
    See all P Glycoprotein primary antibodies

  • Description

    Rabbit monoclonal [EPR10364-57] to P Glycoprotein - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-FoFr, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment aa 350-750. The exact sequence is proprietary.
    Database link: P08183

  • Positive control

    • WB: 293T, HeLa, HepG2 and 293T cell lysates; human fetal brain tissue lysate; mouse brain, heart, kidney and spleen tissue lysates; rat brain, heart, kidney and spleen tissue lysates. IHC-P: Human hepatocellular carcinoma, brain and kidney tissues.

  • General notes

    Ab216656 is the carrier-free version of ab170904. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab216656 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab216656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 141 kDa.

For optimal detection Abcam recommends not boiling the sample after lysis.
IHC-FoFr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for ICC/IF.

    • Target

    • Function

      Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.

    • Tissue specificity

      Expressed in liver, kidney, small intestine and brain.

    • Involvement in disease

      Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.

    • Sequence similarities

      Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • ABC20 antibody
      • ABCB1 antibody
      • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
      • ATP-binding cassette sub-family B member 1 antibody
      • CD243 antibody
      • CLCS antibody
      • Colchicin sensitivity antibody
      • Doxorubicin resistance antibody
      • GP170 antibody
      • MDR1 antibody
      • MDR1_HUMAN antibody
      • Multidrug resistance 1 antibody
      • Multidrug resistance protein 1 antibody
      • P glycoprotein 1 antibody
      • P gp antibody
      • P-glycoprotein 1 antibody
      • PGY1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling P Glycoprotein with purified ab170904 at 1:1200 dilution (0.24 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Hematoxylinwas used as a counterstain.

      Negative control: PBS instead of the primary antibody (inset).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

      Immunohistochemical staining of paraffin embedded human kidney with purified ab170904 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. Counterstained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

      PBS was used instead of the primary antibody as the negative control (inset).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with purified ab170904 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.

      Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue labeling P Glycoprotein with unpurified ab170904 at 1/20. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody.

      Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human kidney tissue labeling P Glycoprotein with unpurified ab170904 at 1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170904).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

      This IHC data was generated using the same anti-P Glycoprotein antibody clone, EPR10364-57, in a different buffer formulation (cat# ab170904).

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling P Glycoprotein with unpurified ab170904 at 1/250 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)
      Western blot - Anti-P Glycoprotein antibody [EPR10364-57] - BSA and Azide free (ab216656)

      This WB data was generated using the same anti-P Glycoprotein antibody clone, EPR10364-57, in a different buffer formulation (cat# ab170904).

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)
      Lane 2: P Glycoprotein knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HepG2 whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - ab170904 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab170904 was shown to specifically react with P Glycoprotein when P Glycoprotein knockout samples were used. Wild-type and P Glycoprotein knockout samples were subjected to SDS-PAGE. Ab170904 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    References

    This product has been referenced in:

    • Fu TG  et al. miR-143 inhibits oncogenic traits by degrading NUAK2 in glioblastoma. Int J Mol Med 37:1627-35 (2016). WB ; Human . Read more (PubMed: 27081712) »
    • Aikawa H  et al. Visualizing spatial distribution of alectinib in murine brain using quantitative mass spectrometry imaging. Sci Rep 6:23749 (2016). IHC-P ; Mouse . Read more (PubMed: 27026287) »
    See all 3 Publications for this product

    Publishing research using ab216656? Please let us know so that we can cite the reference in this datasheet.

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