Overview

  • Product name
    Anti-P Glycoprotein antibody [F4]
    See all P Glycoprotein primary antibodies
  • Description
    Mouse monoclonal [F4] to P Glycoprotein
  • Host species
    Mouse
  • Specificity
    ab80594 detects soluble P glycoprotein in the cultured media of viable adriamycin-resistant cells, whereas those of the drug-sensitive parent cells contain no detectable level of soluble P glycoprotein. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: IHC-P, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Chinese hamster
  • Immunogen

    Human and hamster drug-resistant viable cells followed by crude plasma membranes.

  • Epitope
    Extracellular amino terminal half. This epitope is resistant to formalin fixation and periodate oxidation.
  • Positive control
    • Drug resistant cells. Human kidney or breast carcinoma.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab170904 as a replacement.

Properties

Applications

Our Abpromise guarantee covers the use of ab80594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Fixed-cells only. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • Tissue specificity
    Expressed in liver, kidney, small intestine and brain.
  • Involvement in disease
    Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC20 antibody
    • ABCB1 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
    • ATP-binding cassette sub-family B member 1 antibody
    • CD243 antibody
    • CLCS antibody
    • Colchicin sensitivity antibody
    • Doxorubicin resistance antibody
    • GP170 antibody
    • MDR1 antibody
    • MDR1_HUMAN antibody
    • Multidrug resistance 1 antibody
    • Multidrug resistance protein 1 antibody
    • P glycoprotein 1 antibody
    • P gp antibody
    • P-glycoprotein 1 antibody
    • PGY1 antibody
    see all

References

This product has been referenced in:
  • Zhang HH  et al. Role of 5-Aza-CdR in mitomycin-C chemosensitivity of T24 bladder cancer cells. Oncol Lett 14:5652-5656 (2017). Read more (PubMed: 29142609) »
  • Mesgari Abbasi M  et al. The Effects of Cetirizine on P-glycoprotein Expression and Function In vitro and In situ. Adv Pharm Bull 6:111-8 (2016). Read more (PubMed: 27123426) »

See all 7 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Below is a link to our thorough western blot guide, which includes tips on sample preparation, running, and transfering large proteins.

https://www.abcam.com/ps/pdf/protocols/WB-beginner.pdf

For y...

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I can confirm that it should not be necessary to prepare a membrane fraction in order to detect this protein.

In case you are experiencing high background or to enrich the protein when low signal occurs, I would recommend preparing ...

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Thank you for passing on that reply.

I am sorry to hear that the customer has still not observed the expected signal with the anti-P Glycoprotein antibody (ab3366).

As I mentioned in my previous email, I would be happy to provide the ...

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Thank you for your enquiry.

I can confirm that the epitope of ab80594 Anti-P Glycoprotein antibody [F4] is within the extracellular amino terminal end of the protein.

I hope this will be helpful. If you have any further questions,...

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Thank you for your enquiry and your interest in our products.

Here are a few antibodies against the following targets which have been tested and characterized for Flow cytometry. Please take a look at the on-line datasheets for further inform...

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Thank you for contacting us. ab3364; anti P Glycoprotein is no longer available in our catalogue. However we have similar products available to buy. The catalogue numbers are ab3083; ab3366; ab10333; ab80594; ab80626. Please check the da...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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