Overview

  • Product name
    Anti-P Glycoprotein antibody [JSB-1]
    See all P Glycoprotein primary antibodies
  • Description
    Mouse monoclonal [JSB-1] to P Glycoprotein
  • Host species
    Mouse
  • Specificity
    This antibody is specific for the MDR1 isoform of P Glycoprotein which is only found in the plasma membrane. It does not cross-react with MDR3. It has been shown to cross-react with Pyruvate Carboxylase, an abundant mitochondrial enzyme (130 kDa), on both immunoblots and immunohistochemical tissue sections. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P glycoprotein.
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-P, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Multidrug-resistant Chinese hamster ovary cell line (CHrC5).

  • Epitope
    Cytoplasmic domain present on the MDR1 isoform of P Glycoprotein. This epitope is strongly conserved in mammals.
  • Positive control
    • Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Human liver (positive staining detected along luminal surfaces of bilecanaliculi) or human colon (positive staining localized to luminal surface of secretaoryepithelium).
  • General notes
    This antibody is reported to work in WB according to the literature(PubMed 22389470 and 17961285). Due to customer feedback of difficulty in WB, we are no longer able to guarantee ab3366 in WB.

Properties

Applications

Our Abpromise guarantee covers the use of ab3366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (EDTA, pH 8-9 for example). 20-60 minutes at room temperature.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. PubMed: 18630504

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
  • Tissue specificity
    Expressed in liver, kidney, small intestine and brain.
  • Involvement in disease
    Genetic variations in ABCB1 are associated with susceptibility to inflammatory bowel disease type 13 (IBD13) [MIM:612244]. Inflammatory bowel disease is characterized by a chronic relapsing intestinal inflammation. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. Crohn disease and ulcerative colitis are commonly classified as autoimmune diseases.
  • Sequence similarities
    Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
    Contains 2 ABC transmembrane type-1 domains.
    Contains 2 ABC transporter domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABC20 antibody
    • ABCB1 antibody
    • ATP binding cassette, sub family B (MDR/TAP), member 1 antibody
    • ATP-binding cassette sub-family B member 1 antibody
    • CD243 antibody
    • CLCS antibody
    • Colchicin sensitivity antibody
    • Doxorubicin resistance antibody
    • GP170 antibody
    • MDR1 antibody
    • MDR1_HUMAN antibody
    • Multidrug resistance 1 antibody
    • Multidrug resistance protein 1 antibody
    • P glycoprotein 1 antibody
    • P gp antibody
    • P-glycoprotein 1 antibody
    • PGY1 antibody
    see all

Images

  • ab3366 (4µg/ml) staining p Glycoprotein in human liver, using an automated system (DAKO Autostainer Plus). Using this protocol there is a cell membrane staining of a subpopulation of hepatocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Immunocytochemical analysis of rat spinal cord endothelial cells (RSCECs) labeling P Glycoprotein with ab3366 at 3.35ug/ml.

References

This product has been referenced in:
  • Huang J  et al. Role of gambogic acid and NaI(131) in A549/DDP cells. Oncol Lett 13:37-44 (2017). Human . Read more (PubMed: 28123519) »
  • Huang H  et al. Geniposide reverses multidrug resistance in vitro and in vivo by inhibiting the efflux function and expression of P-glycoprotein. Exp Ther Med 13:437-442 (2017). Read more (PubMed: 28352312) »
See all 26 Publications for this product

Customer reviews and Q&As

1-10 of 52 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

The P glycoprotein antibody ab3366 was used for IHC as shown on the datasheeet with an anti-mouse HRP secondary, then developed with DAB to get the brown color. If you do need an HRP conjugated secondary against mouse, we have many in our catalog including ab98799.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question
Answer

Thank you for contacting us.
The concentration for ab3366, lot GR80263 is 67ug/ml.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you for contacting Abcam.

For ab3366 (https://www.abcam.com/p-glycoprotein-antibody-jsb-1-ab3366.html), the immunogen is a multidrug-resistant Chinese hamster ovary cell line, so the specific epitope is unknown.

ab3366 is the only anti-p glycoprotein antibody that we carry that has been tested and guaranteed to work in the applications (WB, IP, FC, ICC, ELISA, IHC-P) and species (mouse, rat) that you are interested in.

This antibody is covered by our Abpromise guarantee. We are happy to provide scientific support at anytime. If you are using the product in a species and applications listed on the datasheet and contact us within six months of purchase, we are also happy to replace or refund the product should we not be able to help you to resolve the issue. More information on our Abpromise may be found at the following link:
https://www.abcam.com/index.html?pageconfig=abpromise

Our other anti-p glycoprotein antibodies are listed in the link below:
https://www.abcam.com/index.html?t=11041&pt=1

For untested species or applications, we have established a testing discount program. Here is a brief description of how it works:

The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount equal to the value of the product tested. The terms and conditions of this offer can be found here: https://www.abcam.com/AbTrial

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

Read More

Answer

Thank you for passing on that reply.

I am sorry to hear that the customer has still not observed the expected signal with the anti-P Glycoprotein antibody (ab3366).

As I mentioned in my previous email, I would be happy to provide the customer with a different antibody to try. I would suggest that they have a look at the rabbit polyclonal ab129450 or the mouse monoclonal ab80594. Both of these have been used previously and shown to detect the P Glycoprotein in Western blotting.

The use of the mouse monoclonal ab80594 has been described in the following references:

Chu TM et al. Detection of soluble P-glycoprotein in culture media and extracellular fluids. Biochem Biophys Res Commun 203:506-12 (1994). PubMed: 7915520
Chu TM et al. Characterization of a new monoclonal antibody F4 detecting cell surface epitope and P-glycoprotein in drug-resistant human tumor cell lines. Hybridoma 12:417-29 (1993). PubMed: 7503940



Whilst the results observed with the rabbit polyclonal ab129450 can be observed on the datasheet of the antibody:

https://www.abcam.com/ab129450

Although the Western blot for ab129450 is picking up some background, the band of interest is observed strongly and it may well be that with further optimisation of the blocking conditions etc. that the blot would look much cleaner.

Both of these antibodies would again be covered by the Abpromise guarantee were the customer to have any further problems.

I look forward to receiving your reply.

Read More

Answer

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

I have been in contact with the lab concerning this complaint and they have suggested that the antibody may need to be used at a higher concentration in order to see a signal in your Western blot. It may therefore be worthwhile to increase the concentration to 1/10 in order to see if a signal is detected.

However, I can understand your concerns and appreciate the time you have spent in trying to optimise this antibody. If you would like, I can provide you with a different antibody such as the rabbit polyclonal ab129450 or the mouse monoclonal ab80594 to try? Alternatively, if you would prefer, I can arrange for a credit note to be issued.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

Read More

Answer

Thank you for contacting us.

The concentration of this product is 67ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thanks for your reply.

I can confirm this is the same batch number. The full number is 1396975 GR87957-2.

I hope this will be helpful. Please do not hesitate to let me know if you need anything further.

Read More

Answer

Thank you for your enquiry,

I can confirm that the concentration of P Glycoprotein antibody [JSB-1] is lot dependent.

I am sorry I am not sure which batch number you have. The last order we sent to Turkey of this antibody had lot number 1396975 . This has a concentration of 67 ug/ml.

I hope this will be helpful to you. If you check the vial and find you have a different lot number, please do not hesitate to let me know and I will be pleased to find the relevant concentration for you.

Read More

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement of ab3377 with the order number xxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

Read More

Answer

Thank you for this additional information. We strive to offer the best products available and this data is very important for us to be able to maintain the high quality of product which our customers expect.

I will process a free of charge replacement with ab3377 as soon as I receive your Abcam order confirmation number or the PO used to purchase ab3366.

I look forward to your reply. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

1-10 of 52 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up