Product namep-Nitrobenzyl mesylate, Alkylation reagent
Biological descriptionAn alkylation reagent to be used with ab92570 rabbit monoclonal anti-thiophosphate ester antibody and ab138911 ATP-gamma-S for the identification of direct kinase substrates.
Prepare a 50 mM PNBM (12 mg/ml) stock by dissolving PNBM in DMSO and vortexing.
One time use DMSO aliquots may be stored at -20°C.
Chemical name4-Nitrobenzyl mesylate
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab138910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab138910 has been referenced in 13 publications.
- Ducommun S et al. Chemical genetic screen identifies Gapex-5/GAPVD1 and STBD1 as novel AMPK substrates. Cell Signal 57:45-57 (2019). PubMed: 30772465
- Dondelinger Y et al. Serine 25 phosphorylation inhibits RIPK1 kinase-dependent cell death in models of infection and inflammation. Nat Commun 10:1729 (2019). PubMed: 30988283
- Hara M et al. Identification of PNG kinase substrates uncovers interactions with the translational repressor TRAL in the oocyte-to-embryo transition. Elife 7:N/A (2018). PubMed: 29480805
- Shen Y et al. Phosphorylation of CDC25C by AMP-activated protein kinase mediates a metabolic checkpoint during cell-cycle G2/M-phase transition. J Biol Chem 293:5185-5199 (2018). PubMed: 29467227
- Eisenach C et al. ABA-Induced Stomatal Closure Involves ALMT4, a Phosphorylation-Dependent Vacuolar Anion Channel of Arabidopsis. Plant Cell 29:2552-2569 (2017). PubMed: 28874508