p-Nitrobenzyl mesylate, Alkylation reagent (ab138910)
Overview
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Product name
p-Nitrobenzyl mesylate, Alkylation reagent -
Description
Alkylation reagent -
Biological description
An alkylation reagent to be used with ab92570 rabbit monoclonal anti-thiophosphate ester antibody and ab138911 ATP-gamma-S for the identification of direct kinase substrates.
Prepare a 50 mM PNBM (12 mg/ml) stock by dissolving PNBM in DMSO and vortexing.
One time use DMSO aliquots may be stored at -20°C. -
Purity
> 99% -
CAS Number
39628-94-9 -
Chemical structure
Properties
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Chemical name
4-Nitrobenzyl mesylate -
Molecular weight
231.22 -
Molecular formula
C8H9NO5S -
PubChem identifier
18924794 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CS(=O)(=O)OCC1=CC=C(C=C1)[N+](=O)[O-] -
Source
Synthetic
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Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab138910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References
ab138910 has been referenced in 13 publications.
- Ducommun S et al. Chemical genetic screen identifies Gapex-5/GAPVD1 and STBD1 as novel AMPK substrates. Cell Signal 57:45-57 (2019). PubMed: 30772465
- Dondelinger Y et al. Serine 25 phosphorylation inhibits RIPK1 kinase-dependent cell death in models of infection and inflammation. Nat Commun 10:1729 (2019). PubMed: 30988283
- Hara M et al. Identification of PNG kinase substrates uncovers interactions with the translational repressor TRAL in the oocyte-to-embryo transition. Elife 7:N/A (2018). PubMed: 29480805
- Shen Y et al. Phosphorylation of CDC25C by AMP-activated protein kinase mediates a metabolic checkpoint during cell-cycle G2/M-phase transition. J Biol Chem 293:5185-5199 (2018). PubMed: 29467227
- Eisenach C et al. ABA-Induced Stomatal Closure Involves ALMT4, a Phosphorylation-Dependent Vacuolar Anion Channel of Arabidopsis. Plant Cell 29:2552-2569 (2017). PubMed: 28874508
