Key features and details
- Rabbit polyclonal to p107
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-p107 antibody
See all p107 primary antibodies
DescriptionRabbit polyclonal to p107
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
- IHC-P: Human lymph node, testis, colon and kidney tissues. WB: U-251 MG cell lysate. ICC/IF: HeLa cells.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab244504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 121 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionKey regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
Sequence similaritiesBelongs to the retinoblastoma protein (RB) family.
modificationsExists in both phosphorylated and unphosphorylated forms, and T, but not E1A, binds only to the unphosphorylated form. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.
- Information by UniProt
- 107 kDa retinoblastoma-associated protein antibody
- Cellular protein 107 antibody
- Cellular protein p107 antibody
Paraffin-embedded human colon tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.
PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma cells stained for p107 (green) using ab244504 at 4 µg/ml in ICC/IF.
All lanes : Anti-p107 antibody (ab244504) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Predicted band size: 121 kDa
Paraffin-embedded human cerebral cortex tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human testis tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human lymph node tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human pancreas tissue stained for p107 using ab244504 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab244504 has not yet been referenced specifically in any publications.