Overview

  • Product name

  • Description

    Rabbit polyclonal to p107
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human p107 aa 1018-1068. The exact sequence is proprietary.
    Database link: P28749

  • Positive control

    • IP: p107 IP in Jurkat whole cell lysate.
  • General notes

    ab245514 has not performed satisfactorily when used for WB of p107 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of p107.

Properties

Applications

Our Abpromise guarantee covers the use of ab245514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.

8 - 15 µl/mg 

  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
    • Sequence similarities

      Belongs to the retinoblastoma protein (RB) family.
    • Post-translational
      modifications

      Exists in both phosphorylated and unphosphorylated forms, and T, but not E1A, binds only to the unphosphorylated form. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 107 kDa retinoblastoma-associated protein antibody
      • Cellular protein 107 antibody
      • Cellular protein p107 antibody
      • CP107 antibody
      • MGC40006 antibody
      • p107 antibody
      • PRB1 antibody
      • RBL1 antibody
      • RBL1_HUMAN antibody
      • Retinoblastoma like protein 1 antibody
      • retinoblastoma-like 1 (p107) antibody
      • Retinoblastoma-like protein 1 antibody
      see all

    Images

    • p107 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) using ab245514 at 8 µl per reaction. Western blot was performed on the immunoprecipitate using ab245514 at 1/400 dilution.

      Lane 1: ab245514 IP in Jurkat cell lysate.
      Lane 2: Control IgG IP in Jurkat cell lysate.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

    References

    ab245514 has not yet been referenced specifically in any publications.

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