Key features and details
- Mouse monoclonal [OTI4A10] to p107
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-p107 antibody [OTI4A10]
See all p107 primary antibodies
DescriptionMouse monoclonal [OTI4A10] to p107
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to Human p107 aa 739-1014. Produced in E.coli (NP_899662).
MNTNQESKVKSPVSLTAHSLIGASPKQTNLTKAQEVHSTGINRPKRTGSL ALFYRKVYHLASVRLRDLCLKLDVSNELRRKIWTCFEFTLVHCPDLMKDR HLDQLLLCAFYIMAKVTKEERTFQEIMKSYRNQPQANSHVYRSVLLKSIP REVVAYNKNINDDFEMIDCDLEDATKTPDCSSGPVKEERGDLIKFYNTIY VGRVKSFALKYDLANQDHMMDAPPLSPFPHIKQQPGSPRRISQQHSIYIS PHKNGSGLTPRSALLYKFNGSPSKVR
Database link: P28749-2
- WB: pCMV6-ENTRY p107 cDNA transfected HEK-293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 114, 121 kDa.|
FunctionKey regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor.
Sequence similaritiesBelongs to the retinoblastoma protein (RB) family.
modificationsExists in both phosphorylated and unphosphorylated forms, and T, but not E1A, binds only to the unphosphorylated form. Cell-cycle arrest properties are inactivated by phosphorylation on Thr-332, Ser-640, Ser-964 and Ser-975 by CDK4.
- Information by UniProt
- 107 kDa retinoblastoma-associated protein antibody
- Cellular protein 107 antibody
- Cellular protein p107 antibody
All lanes : Anti-p107 antibody [OTI4A10] (ab236518) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY p107 cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 114, 121 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236518 has not yet been referenced specifically in any publications.