Anti-p114RhoGEF antibody (ab10152)
Key features and details
- Goat polyclonal to p114RhoGEF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p114RhoGEF antibody
See all p114RhoGEF primary antibodies -
Description
Goat polyclonal to p114RhoGEF -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide: SAKEDASKEDVIFF, corresponding to C terminal amino acids 1037-1050 of Human p114RhoGEF.
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General notes
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
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| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration.
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
WB
Use at an assay dependent concentration. 1 hour primary incubation is recommended for this product. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 23370 Human
- SwissProt: Q6ZSZ5 Human
- Unigene: 465761 Human
- Unigene: 736818 Human
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Alternative names
- 114 kDa Rho specific guanine nucleotide exchange factor antibody
- 114 kDa Rho-specific guanine nucleotide exchange factor antibody
- ARHGEF 18 antibody
see all
Images
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Western blot - Anti-p114RhoGEF antibody (ab10152)All lanes : Anti-p114RhoGEF antibody (ab10152) at 0.5 µg/ml
Lane 1 : Daudi cell lysate (protein in RIPA buffer)
Lane 2 : Caco-2 cell lysate (protein in RIPA buffer)
Lysates/proteins at 35 µg per lane.Detected by chemiluminescence.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab10152 has been referenced in 2 publications.
- Acharya BR et al. A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress. Dev Cell 47:439-452.e6 (2018). PubMed: 30318244
- Tsuji T et al. Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells. Mol Biol Cell 21:3590-600 (2010). PubMed: 20810787