Anti-p114RhoGEF antibody (ab10152)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-p114RhoGEF antibody
See all p114RhoGEF primary antibodies -
DescriptionGoat polyclonal to p114RhoGEF
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Host speciesGoat
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Tested applicationsSuitable for: WB, IP, ICC/IFmore details
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Species reactivityReacts with: Dog, Human
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Immunogen
Synthetic peptide: SAKEDASKEDVIFF, corresponding to C terminal amino acids 1037-1050 of Human p114RhoGEF.
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Positive control
- Human muscle and HepG2 extracts. Caco-2, HCE and MDCK cells.
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General notes
Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Primary antibody notesRho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab10152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Can be blocked with Human p114RhoGEF peptide (ab23189). | |
| IP | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 23370 Human
- SwissProt: Q6ZSZ5 Human
- Unigene: 465761 Human
- Unigene: 736818 Human
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Alternative names
- 114 kDa Rho specific guanine nucleotide exchange factor antibody
- 114 kDa Rho-specific guanine nucleotide exchange factor antibody
- ARHGEF 18 antibody
see all
Images
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Western blot - Anti-p114RhoGEF antibody (ab10152)All lanes : Anti-p114RhoGEF antibody (ab10152) at 0.5 µg/ml
Lane 1 : Daudi cell lysate (protein in RIPA buffer)
Lane 2 : Caco-2 cell lysate (protein in RIPA buffer)
Lysates/proteins at 35 µg per lane.Detected by chemiluminescence.
Protocols
References
This product has been referenced in:
- Tsuji T et al. Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells. Mol Biol Cell 21:3590-600 (2010). Read more (PubMed: 20810787) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"