Overview

  • Product name

  • Description

    Goat polyclonal to p114RhoGEF
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Dog, Human
  • Immunogen

    Synthetic peptide: SAKEDASKEDVIFF, corresponding to C terminal amino acids 1037-1050 of Human p114RhoGEF.

  • Positive control

    • Human muscle and HepG2 extracts. Caco-2, HCE and MDCK cells.
  • General notes


    Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes

    Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription and cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). p114RhoGEF is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • 114 kDa Rho specific guanine nucleotide exchange factor antibody
    • 114 kDa Rho-specific guanine nucleotide exchange factor antibody
    • ARHGEF 18 antibody
    • Arhgef18 antibody
    • ARHGI_HUMAN antibody
    • KIAA0521 antibody
    • MGC15913 antibody
    • p114 Rho GEF antibody
    • p114-Rho-GEF antibody
    • p114RhoGEF antibody
    • Rho guanine nucleotide exchange factor 18 antibody
    • Rho specific guanine nucleotide exchange factor p114 antibody
    • Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody
    • Rho/Rac guanine nucleotide exchange factor 18 antibody
    • SA RhoGEF antibody
    • SA-RhoGEF antibody
    • Septin associated RhoGEF antibody
    • Septin-associated RhoGEF antibody
    see all

Images

  • All lanes : Anti-p114RhoGEF antibody (ab10152) at 0.5 µg/ml

    Lane 1 : Daudi cell lysate (protein in RIPA buffer)
    Lane 2 : Caco-2 cell lysate (protein in RIPA buffer)

    Lysates/proteins at 35 µg per lane.


    Detected by chemiluminescence.

References

This product has been referenced in:

  • Tsuji T  et al. Involvement of p114-RhoGEF and Lfc in Wnt-3a- and dishevelled-induced RhoA activation and neurite retraction in N1E-115 mouse neuroblastoma cells. Mol Biol Cell 21:3590-600 (2010). Read more (PubMed: 20810787) »
See 1 Publication for this product

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