Overview

  • Product name

  • Description

    Rabbit polyclonal to p114RhoGEF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 612-802 of Human ARHGEF18 (NP_056133)

  • Positive control

    • WB: HepG2 whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: Isoform 1, 131 ; Isoform 2, 114 kDa.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • 114 kDa Rho specific guanine nucleotide exchange factor antibody
    • 114 kDa Rho-specific guanine nucleotide exchange factor antibody
    • ARHGEF 18 antibody
    • Arhgef18 antibody
    • ARHGI_HUMAN antibody
    • KIAA0521 antibody
    • MGC15913 antibody
    • p114 Rho GEF antibody
    • p114-Rho-GEF antibody
    • p114RhoGEF antibody
    • Rho guanine nucleotide exchange factor 18 antibody
    • Rho specific guanine nucleotide exchange factor p114 antibody
    • Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody
    • Rho/Rac guanine nucleotide exchange factor 18 antibody
    • SA RhoGEF antibody
    • SA-RhoGEF antibody
    • Septin associated RhoGEF antibody
    • Septin-associated RhoGEF antibody
    see all

Images

  • ICC/IF image of ab96520 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab96520 at 5µg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-p114RhoGEF antibody (ab96520) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: Isoform 1, 131 ; Isoform 2, 114 kDa



    7.5% SDS-PAGE

References

This product has been referenced in:

  • Hu J  et al. MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1. PLoS One 8:e68291 (2013). WB ; Human . Read more (PubMed: 23826386) »
See 1 Publication for this product

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