Key features and details
- Mouse monoclonal [OTI6D10] to p114RhoGEF
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-p114RhoGEF antibody [OTI6D10]
See all p114RhoGEF primary antibodies
DescriptionMouse monoclonal [OTI6D10] to p114RhoGEF
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human p114RhoGEF aa 118-215. NP_001124427. Produced in E. coli.
Database link: Q6ZSZ5
- WB: pCMV6-ENTRY p114RhoGEF transfected HEK-293T cell lysate. HepG2 and HeLa cell extracts. IHC-P: Human thyroid carcinoma, lymph node and breast tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 131 kDa.|
|IHC-P||1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
- Information by UniProt
- 114 kDa Rho specific guanine nucleotide exchange factor antibody
- 114 kDa Rho-specific guanine nucleotide exchange factor antibody
- ARHGEF 18 antibody
All lanes : Anti-p114RhoGEF antibody [OTI6D10] (ab236374) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell extracts
Lysates/proteins at 35 µg per lane.
Predicted band size: 131 kDa
Paraffin-embedded human breast tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human thyroid carcinoma tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human lymph node tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.
All lanes : Anti-p114RhoGEF antibody [OTI6D10] (ab236374) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY p114RhoGEF transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 131 kDa
Cells transfected for 48 hours then lysed.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236374 has not yet been referenced specifically in any publications.