Overview

  • Product name

    Anti-p114RhoGEF antibody [OTI6D10]
    See all p114RhoGEF primary antibodies
  • Description

    Mouse monoclonal [OTI6D10] to p114RhoGEF
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human p114RhoGEF aa 118-215. NP_001124427. Produced in E. coli.
    Database link: Q6ZSZ5

  • Positive control

    • WB: pCMV6-ENTRY p114RhoGEF transfected HEK-293T cell lysate. HepG2 and HeLa cell extracts. IHC-P: Human thyroid carcinoma, lymph node and breast tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    OTI6D10
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 131 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Information by UniProt
  • Database links

  • Alternative names

    • 114 kDa Rho specific guanine nucleotide exchange factor antibody
    • 114 kDa Rho-specific guanine nucleotide exchange factor antibody
    • ARHGEF 18 antibody
    • Arhgef18 antibody
    • ARHGI_HUMAN antibody
    • KIAA0521 antibody
    • MGC15913 antibody
    • p114 Rho GEF antibody
    • p114-Rho-GEF antibody
    • p114RhoGEF antibody
    • Rho guanine nucleotide exchange factor 18 antibody
    • Rho specific guanine nucleotide exchange factor p114 antibody
    • Rho/Rac guanine nucleotide exchange factor (GEF) 18 antibody
    • Rho/Rac guanine nucleotide exchange factor 18 antibody
    • SA RhoGEF antibody
    • SA-RhoGEF antibody
    • Septin associated RhoGEF antibody
    • Septin-associated RhoGEF antibody
    see all

Images

  • All lanes : Anti-p114RhoGEF antibody [OTI6D10] (ab236374) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extracts
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell extracts

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 131 kDa

  • Paraffin-embedded human breast tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human thyroid carcinoma tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human lymph node tissue stained for p114RhoGEF with ab236374 at a 1/500 dilution in immunohistochemical analysis.

  • All lanes : Anti-p114RhoGEF antibody [OTI6D10] (ab236374) at 1/2000 dilution

    Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : pCMV6-ENTRY p114RhoGEF transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 131 kDa



    Cells transfected for 48 hours then lysed.

References

ab236374 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236374.
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