Key features and details
- Rabbit polyclonal to p115-RhoGEF
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-p115-RhoGEF antibody
See all p115-RhoGEF primary antibodies
DescriptionRabbit polyclonal to p115-RhoGEF
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- WB: RT-4 and U-251 MG cell lysates. ICC/IF: A549 cells.
Previously labelled as ARHGEF1.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab220892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 102 kDa.|
FunctionSeems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
Tissue specificityUbiquitously expressed.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 RGSL (RGS-like) domain.
DomainThe RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.
The DH domain is involved in interaction with CCPG1.
modificationsPhosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2.
Cellular localizationCytoplasm. Membrane. Translocated to the membrane by activated GNA13 or LPA stimulation.
- Information by UniProt
- 115 kD protein antibody
- 115 kDa guanine nucleotide exchange factor antibody
- ARHG1_HUMAN antibody
All lanes : Anti-p115-RhoGEF antibody (ab220892) at 1/100 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Predicted band size: 102 kDa
Immunofluorescent analysis of PFA fixed, triton X-100 permeabilized A549 cells labeling p115-RhoGEF using ab220882 at 4 μg/mL (green).
ab220892 has been referenced in 1 publication.
- Fu J et al. 1, 6-di-O-caffeoyl-ß-D-glucopyranoside, a natural compound from Callicarpa nudiflora Hook impairs P2Y12 and thromboxane A2 receptor-mediated amplification of platelet activation and aggregation. Phytomedicine 36:273-282 (2017). PubMed: 29157825