Anti-p13 antibody [BM3] (ab1044)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-p13 antibody [BM3] -
Description
Mouse monoclonal [BM3] to p13 -
Host species
Mouse -
Specificity
This protein A purified mouse monoclonal antibody reacts specifically with p13 in human granulocytes and monocytes residing in lymphoid and non-lymphoid tissues. The antibody recognizes a 13 kDa band corresponding to p13 -
Tested applications
Suitable for: Flow Cyt, IHC-Frmore details -
Species reactivity
Reacts with: Human -
Immunogen
A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes
-
General notes
BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13 kDa protein.
The antibody does not stain tissue after paraffin treatment.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13 kDa protein. -
Clonality
Monoclonal -
Clone number
BM3 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab1044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
| IHC-Fr | 1/100. |
Target
-
Relevance
p13 is a specific marker for human myeloid cells. Induction studies using HL-60 cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues.
Datasheets and documents
References
This product has been referenced in:
- Asadi A et al. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem 63:646-62 (2015). Read more (PubMed: 26216140) »
