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    p13-antibody-bm3-ab1044.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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Anti-p13 antibody [BM3] (ab1044)

  • Datasheet
  • SDS
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Key features and details

  • Mouse monoclonal [BM3] to p13
  • Reacts with: Human
  • Isotype: IgG1

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Secondary
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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Overview

  • Product name

    Anti-p13 antibody [BM3]
  • Description

    Mouse monoclonal [BM3] to p13
  • Host species

    Mouse
  • Specificity

    This protein A purified mouse monoclonal antibody reacts specifically with p13 in human granulocytes and monocytes residing in lymphoid and non-lymphoid tissues. The antibody recognizes a 13 kDa band corresponding to p13
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes

  • General notes

    BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13 kDa protein.

     

    The antibody does not stain tissue after paraffin treatment.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Primary antibody notes

    BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13 kDa protein.
  • Clonality

    Monoclonal
  • Clone number

    BM3
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Target

  • Relevance

    p13 is a specific marker for human myeloid cells. Induction studies using HL-60 cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues.

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab1044? Please let us know so that we can cite the reference in this datasheet.

ab1044 has been referenced in 1 publication.

  • Asadi A  et al. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem 63:646-62 (2015). PubMed: 26216140

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