Overview

  • Product name
    Anti-p13 antibody [BM3]
  • Description
    Mouse monoclonal [BM3] to p13
  • Host species
    Mouse
  • Specificity
    This protein A purified mouse monoclonal antibody reacts specifically with p13 in human granulocytes and monocytes residing in lymphoid and non-lymphoid tissues. The antibody recognizes a 13 kDa band corresponding to p13
  • Tested applications
    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A BALB/c mouse was immunized with nuclei from Pokeweed mitogen stimulated human peripheral blood lymphocytes

  • General notes

    BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13 kDa protein.

     

    The antibody does not stain tissue after paraffin treatment.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • Purity
    Protein A purified
  • Primary antibody notes
    BM3 is an early marker of myeloid differentiation. Immunoprecipitation experiments using S35 methionine labeled human myeloid leukemia cells show that BM3 identifies a 13 kDa protein.
  • Clonality
    Monoclonal
  • Clone number
    BM3
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-Fr 1/100.

Target

  • Relevance
    p13 is a specific marker for human myeloid cells. Induction studies using HL-60 cells show that p13 is expressed during the early phases of myeloid differentiation. The mAb anti-p13 recognizes a nuclear antigen expressed in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues.

References

This product has been referenced in:
  • Asadi A  et al. Characterization of Antibodies to Products of Proinsulin Processing Using Immunofluorescence Staining of Pancreas in Multiple Species. J Histochem Cytochem 63:646-62 (2015). Read more (PubMed: 26216140) »

See 1 Publication for this product

Publishing research using ab1044? Please let us know so that we can cite the reference in this datasheet.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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